| 產(chǎn)品編號 | bs-42393R |
| 英文名稱 | Rabbit Anti-MADCAM1 antibody |
| 中文名稱 | 粘膜細(xì)胞粘附分子1抗體 |
| 別 名 | Addressin mucosal; hMAdCAM 1; hMAdCAM-1; hMAdCAM1; MACAM1; MADCA_HUMAN; MAdCAM 1; MAdCAM-1; Madcam1; Mucosal addressin cell adhesion molecule 1; Mucosal vascular addressin cell adhesion molecule 1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant mouse MADCAM1 protein: 22-364/405 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The recirculation of lymphocytes through different organs is thought to be regulated by adhesion molecules recognizing tissue-specific vascular addressins on the endothelium. The mucosal vascular addressin, MadCAM-1 (mucosal addressin cell adhesion molecule 1), is an immunoglobulin superfamily adhesion molecule for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into Peyer’s patches and the intestinal lamina propria. MadCAM-1 acts as an endothelial cell ligand for leukocyte homing receptors L-Selectin and Integrin Alpha 4/Beta 7. MadCAM-1 is strongly expressed on inflamed portal vein/sinusoidal endothelium in autoimmune-mediated liver disease and plays a major contributory role in the progression of chronic experimental autoimmune encephalomyelitis. Function: Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. Subunit: Contains 2 Ig-like (immunoglobulin-like) domains. Subcellular Location: Membrane. Tissue Specificity: Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes. Post-translational modifications: The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation (By similarity). Similarity: Contains 2 Ig-like (immunoglobulin-like) domains. SWISS: Q61826 Gene ID: 17123 Database links: MAdCAM-1（粘膜血管定居因子或黏膜地址素細(xì)胞黏附分子）、CD44等均參與癌癥和腫瘤細(xì)胞與血管內(nèi)皮的相互作用，與癌癥和腫瘤細(xì)胞一起穿過血管內(nèi)皮而進(jìn)入血管內(nèi)，參與血行播散轉(zhuǎn)移。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with MADCAM1 polyclonal antibody, unconjugated (bs-42393R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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