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Rabbit Anti-CD144/VE Cadherin  antibody (bs-0878R)  
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產(chǎn)品編號 bs-0878R
英文名稱 Rabbit Anti-CD144/VE Cadherin  antibody
中文名稱 血管內(nèi)皮鈣粘蛋白抗體
別    名 VE-cadherin; 7B4 antigen; VECD; Vascular endothelial cell cadherin; 7B 4; 7B4; 7B4 antigen; Cadherin 5; Cadherin 5 type 2; Cadherin-5; Cadherin5; CD 144; CD144 antigen; CDH 5; CDH5; CDH5 protein; Vascular endothelial cadherin; VE Cad antibody VEC; CADH5_HUMAN.  
Specific References  (16)     |     bs-0878R has been referenced in 16 publications.
[IF=16.874] Bingcheng Yi. et al. Step-wise CAG@PLys@PDA-Cu2+ modification on micropatterned nanofibers for programmed endothelial healing. BIOACT MATER. 2022 Jul;:  IF ;  Human.  
[IF=15.304] Xin Liu. et al. Construction of functional magnetic scaffold with temperature control switch for long-distance vascular injury. BIOMATERIALS. 2022 Oct;:121862  IF ;  Mouse.  
[IF=10.269] Yi, Bingcheng. et al. Soft nanofiber modified micropatterned substrates enhance native-like endothelium maturation via CXCR4/calcium-mediated actin cytoskeleton assembly. NANO RES. 2022 Aug;:1-18  IF ;  Human.  
[IF=7.109] Xiong, Jingjie. et al. CircRNA mmu_circ_0000021 regulates microvascular function via the miR-143-3p/NPY axis and intracellular calcium following ischemia/reperfusion injury. CELL DEATH DISCOV. 2022 Jul;8(1):1-13  IHC ;  Mouse.  
[IF=5.714] Li-Juan You. et al. Schisandrin A ameliorates increased pulmonary capillary endothelial permeability accompanied with sepsis through inhibition of RhoA/ROCK1/MLC pathways. INT IMMUNOPHARMACOL. 2023 May;118:110124  WB ;  Rat,Human.  
[IF=5.57] Zhang C et al. Superparamagnetic iron oxide (SPIO) nanoparticles labeled endothelial progenitor cells (EPCs) administration inhibited heterotopic ossification in rats. Nanomedicine. 2019 Aug 7;21:102078.  IHC-P&WB ;  Rat.  
[IF=5.502] Shunnosuke Kunoki. et al. Inhibition of transglutaminase 2 reduces peritoneal injury in a chlorhexidine-induced peritoneal fibrosis model. LAB INVEST. 2023 Jan;:100050  IF ;  Rat.  
[IF=5.344] Ling-Kun Zhang. et al. Growth factors regional patterned and photoimmobilized scaffold applied to bone tissue regeneration. J Mater Chem B. 2020 Dec;8(48):10990-11000  IHC ;  Rat.  
[IF=4.932] Yi Ren. et al. Pravastatin attenuates sepsis-induced acute lung injury through decreasing pulmonary microvascular permeability via inhibition of Cav-1/eNOS pathway. Int Immunopharmacol. 2021 Nov;100:108077  IHC ;  Mouse.  
[IF=4.225] Liu Yi. et al. Overexpression of Activating Transcription Factor 3 Alleviates Cardiac Microvascular Ischemia/Reperfusion Injury in Rats. Front Pharmacol. 2021 Feb;12:71  IF,IHC ;  Rat.  
[IF=3.118] Xia W et al. Inhibition of MRP4 alleviates sepsis-induced acute lung injury in rats. Int Immunopharmacol. 2019 Apr 14;72:211-217.  WB ;  Rat.  
[IF=3.115] Wan‐Ting Hung. et al. Leptin protects brain from ischemia/reperfusion‐induced infarction by stabilizing the blood–brain barrier to block brain infiltration by the blood‐borne neutrophils. Eur J Neurosci. 2020 Dec;52(12):4890-4907  IHC ;  Rat.  
[IF=2.766] Bhagwani AR et al. Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension.PLoS One.?2019 Mar 18;14(3):e0213890.  FCM ;  Rat.  
[IF=1.829] Zhong Y et al. Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines.Stem Cell Res. 2018 Jul;30:100-112.  ICF ;  Mouse.  
[IF=1.702] Dai X et al. Effect of Aerobic and Resistance Training on Endothelial Progenitor Cells in Mice with Type 2 Diabetes. Cell Reprogram . 2020 Aug;22(4):189-197.  IF ;  mouse.  
[IF=0.833] K?nig?ML et al. Validation of a human angiopoietin-2 ELISA for measurement of angiopoietin-2 concentrations in canine plasma samples and supernatant of primary canine aortic endothelial cell cultures. Am J Vet Res. 2018 Aug;79(8):803-810.  ICF ;  Dog.  
研究領域 腫瘤  心血管  免疫學  信號轉導  細胞粘附分子  血管內(nèi)皮細胞  上皮細胞  內(nèi)皮細胞  細胞外基質  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human
產(chǎn)品應用 WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 86kDa
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse Vascular endothelial cell cadherin: 601-700/784 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008].

Function:
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.

Subunit:
Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1.

Subcellular Location:
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.

Tissue Specificity:
Endothelial tissues and brain.

Post-translational modifications:
Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.

Similarity:
Contains 5 cadherin domains.

SWISS:
P55284

Gene ID:
12562

Database links:

Entrez Gene: 1003 Human

Entrez Gene: 12562 Mouse

Entrez Gene: 307618 Rat

Omim: 601120 Human

SwissProt: P33151 Human

SwissProt: P55284 Mouse

Unigene: 76206 Human

Unigene: 21767 Mouse

Unigene: 224644 Rat



血管內(nèi)皮鈣粘蛋白(Vascularendothelial cell cadherin;VE-cadherin;VECD),又名CD144,Cadherin-5,是AJ連接(粘附連接)的主要粘附分子,是維持血管內(nèi)皮細胞極性和完整性必不可少的內(nèi)皮細胞特異性鈣粘蛋白.
產(chǎn)品圖片
Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: HUVEC (Human) Cell Lysate at 30 ug Lane 3: A431 (Human) Cell Lysate at 30 ug Lane 4: LOVO (Human) Cell Lysate at 30 ug Lane 5: SiHa (Human) Cell Lysate at 30 ug Primary: Anti-CD144/VE Cadherin (bs-0878R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD
Blank control:HUVEC. Primary Antibody (green line): Rabbit Anti-CD144/VE Cadherin antibody (bs-0878R) Dilution: 1ug/Test; Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line):Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:HUVEC. Primary Antibody (green line): Rabbit Anti-CD144/VE Cadherin antibody (bs-0878R) Dilution: 1ug/Test; Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line):Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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