| 產(chǎn)品編號(hào) | bs-0556R |
| 英文名稱 | Rabbit Anti-HMGA2 antibody |
| 中文名稱 | 高遷移率族蛋白A2抗體 |
| 別 名 | HMGA 2; BABL; High mobility group (nonhistone chromosomal) protein isoform I C; High mobility group (nonhistone chromosomal) protein isoform IC; High Mobility Group AT hook 2; High Mobility Group AT hook protein 2; High mobility group protein HMGI C; High mobility group protein HMGIC; High mobility group protein I, isoform C; HMGA 2; HMGI C; HMGIC; LIPO; Non histone chromosomal architectural protein HMGI C; Non histone chromosomal architectural protein HMGIC; Pygmy; STQTL9; HMGA2_HUMAN. |
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Specific References (2) | bs-0556R has been referenced in 2 publications.
[IF=3.146] Hou, Xiaocan, et al. "Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression." American Journal of Translational Research 8.9 (2016): 3656. WB ; Mouse.
[IF=2.976] Yang Y et al. MicroRNA-33b-5p is overexpressed and inhibits GLUT4 by targeting HMGA2 in polycystic ovarian syndrome: An in vivo and in vitro study.Oncol Rep. 2018 Jun;39(6):3073-3085. IHC-P&WB ; Rat&Human.
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| 研究領(lǐng)域 | 腫瘤 結(jié)合蛋白 細(xì)胞類型標(biāo)志物 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Chicken) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HMGA2: 41-109/109 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Function: Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Subunit: Interacts with E4F1. Interacts with NEK2 (By similarity). Subcellular Location: Nucleus. Post-translational modifications: Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. Phosphorylated by NEK2. DISEASE: Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas. Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas. Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma. Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51B. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma. Similarity: Belongs to the HMGA family. Contains 3 A.T hook DNA-binding domains. SWISS: P52926 Gene ID: 8091 Database links: Entrez Gene: 8091 Human Entrez Gene: 15364 Mouse Omim: 600698 Human SwissProt: P52926 Human SwissProt: P52927 Mouse Unigene: 505924 Human Unigene: 157190 Mouse HMGA-2高遷移率蛋白A-2：也是目前特異腫瘤標(biāo)記物，主要用于腫瘤方面的研究。 |
| 產(chǎn)品圖片 |
Tissue/cell: human ovary carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HMGA2 Polyclonal Antibody, Unconjugated(bs-0556R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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