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Rabbit Anti-KLF4  antibody (bs-1064R)  
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產(chǎn)品編號 bs-1064R
英文名稱 Rabbit Anti-KLF4  antibody
中文名稱 腸道內(nèi)富含的Kruppel樣因子/上皮鋅指蛋白4抗體
別    名 Endothelial Kruppel like zinc finger protein; EZF; GKLF; KLF; Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor); Kruppel like factor 4 (gut); Krueppel-like factor 4;EZF;GKLF;KLF 4;KLF-4;Krueppel like factor 4; KLF4_HUMAN.  
Specific References  (9)     |     bs-1064R has been referenced in 9 publications.
[IF=7.525] Ma Manting. et al. LncRNA-TBP mediates TATA-binding protein recruitment to regulate myogenesis and induce slow-twitch myofibers. CELL COMMUN SIGNAL. 2023 Dec;21(1):1-16  WB ;  Chicken.  
[IF=6.656] Dong-qin Xu. et al. Pentoxifylline attenuates nonalcoholic fatty liver by inhibiting hepatic macrophage polarization to the M1 phenotype. PHYTOMEDICINE. 2022 Aug;:154368  FC ;  Mouse.  
[IF=6.639] Wataru Nakajima. et al. Epigenetic Priming with Decitabine Augments the Therapeutic Effect of Cisplatin on Triple-Negative Breast Cancer Cells through Induction of Proapoptotic Factor NOXA. Cancers. 2022 Jan;14(1):248  WB ;  Human.  
[IF=6.208] Ling Ding. et al. Bufalin Inhibits Tumorigenesis, Stemness, and Epithelial–Mesenchymal Transition in Colorectal Cancer through a C-Kit/Slug Signaling Axis. INT J MOL SCI. 2022 Jan;23(21):13354  WB ;  Human.  
[IF=5.81] Yuqiao Yang. et al. Oxytocin Protects Against Isoproterenol-Induced Cardiac Hypertrophy by Inhibiting PI3K/AKT Pathway via a lncRNA GAS5/miR-375-3p/KLF4-Dependent Mechanism. Front Pharmacol. 2021; 12: 766024  WB ;  Rat.  
[IF=3.759] Wataru Nakajima. et al. Krüppel-Like Factor 4 and Its Activator APTO-253 Induce NOXA-Mediated, p53-Independent Apoptosis in Triple-Negative Breast Cancer Cells. Genes-Basel. 2021 Apr;12(4):539  WB ;  Human.  
[IF=3.2] El-Sayed, Ahmed Kamel, et al. "Pluripotent state induction in mouse embryonic fibroblast using mRNAs of reprogramming factors." International journal of molecular sciences 15.12 (2014): 21840-21864.  IF(ICC) ;  Mouse.  
[IF=2.76] Verma, Subodh, et al. ?Pedicled no-touch saphenous vein graft harvest limits vascular smooth muscle cell activation: the PATENT saphenous vein graft study.? European Journal of Cardio-Thoracic Surgery (2013): ezt560.  IHC-P ;  Human.  
[IF=1.448] Wang Q et al. KLF4 overexpression decreases the viability, invasion and migration of papillary thyroid cancer cells. EXPERIMENTAL AND THERAPEUTIC MEDICINE.2019 18: 3493-3501.  IHC-P ;  Human.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  信號轉(zhuǎn)導(dǎo)  干細(xì)胞  細(xì)胞周期蛋白  轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1ug/Test,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 56kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human KLF4: 415-513/513 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.

Function:
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

Subunit:
Interacts with POU5F1/OCT4 and SOX2. Interacts with MUC1 (via the C-terminal domain).

Subcellular Location:
Nucleus.

Post-translational modifications:
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.

SWISS:
O43474

Gene ID:
9314

Database links:

Entrez Gene: 9314 Human

Entrez Gene: 16600 Mouse

Omim: 602253 Human

SwissProt: O43474 Human

SwissProt: Q60793 Mouse

Unigene: 376206 Human

Unigene: 4325 Mouse



Klf4(原稱GKLF)調(diào)節(jié)體外細(xì)胞的增生與分化,有學(xué)者認(rèn)為:KLF4可能參與消化系統(tǒng)腫瘤的發(fā)生及發(fā)展過程,多表達與結(jié)腸。又稱:上皮鋅指轉(zhuǎn)錄因子(上皮鋅指蛋白)Surface skin layer zinc finger protein。
產(chǎn)品圖片
Sample: 293T (human)Cell Lysate at 40 ug Primary: Anti- KLF4 (bs-1064R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 60 kD
Sample: Lane 1: Mouse NIH/3T3 cell lysates Lane 2: Human A431 cell lysates Lane 3: Human LOVO cell lysates Lane 4: Human HeLa cell lysates Lane 5: Human MCF-7 cell lysates Lane 6: Human A549 cell lysates Primary: Anti-KLF4 (bs-1064R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 50 kDa
Sample: Hela Cell(Human)Lysate at 30 ug Primary: Anti- KLF4 (bs-1064R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56kD Observed band size: 56kD
Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLF4) Polyclonal Antibody, Unconjugated (bs-1064R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KLF4 Polyclonal Antibody, Unconjugated(bs-1064R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: MCF7(blue). Primary Antibody: Rabbit Anti-KLF4 AF647 Conjugated antibody (bs-1064R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-1064R-AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control: K562. Primary Antibody (green line): Rabbit Anti-KLF4 antibody (bs-1064R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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