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Rabbit Anti-N-cadherin antibody (bs-1172R)

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產(chǎn)品編號(hào)	bs-1172R
英文名稱	Rabbit Anti-N-cadherin antibody
中文名稱	N-鈣粘附分子抗體
別名	Cadherin 2; Cadherin 2 N cadherin neuronal; Cadherin 2 type 1; Cadherin 2 type 1 N cadherin neuronal; cadherin 2 type 1 N-cadherin neuronal; Cadherin2; Calcium dependent adhesion protein neuronal; CD325; CD325 antigen; CDH2; CDHN; CDw325; CDw325 antigen; N cadherin 1; NCAD; Neural Cadherin; Neural Cadherin.
	Specific References (33) \| bs-1172R has been referenced in 33 publications. [IF=9.933] Xingyi Xu. et al. A Honeycomb-Like Bismuth/Manganese Oxide Nanoparticle with Mutual Reinforcement of Internal and External Response for Triple-Negative Breast Cancer Targeted Therapy. 2021 Jul 23 WB ; Human. PubMed:34297897 [IF=9.584] Zheng, Bingxin. et al. Siglec-15-induced autophagy promotes invasion and metastasis of human osteosarcoma cells by activating the epithelial–mesenchymal transition and Beclin-1/ATG14 pathway. CELL BIOSCI. 2022 Dec;12(1):1-15 WB ; Human. PubMed:35842729 [IF=8.456] Zhang D et al. Cell Membrane-Coated Porphyrin Metal-Organic Frameworks for Cancer Cell Targeting and O2-Evolving Photodynamic Therapy. ACS Appl Mater Interfaces. 2019 Oct 30;11(43):39594-39602. WB ; Human. PubMed:31577410 [IF=7.727] Xue Wang. et al. Engineered liposomes targeting the gut–CNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:390 IF ; Rat. PubMed:33485884 [IF=7.076] Mengmeng Niu. et al. Noncanonical TGF-β signaling leads to FBXO3-mediated degradation of ΔNp63α promoting breast cancer metastasis and poor clinical prognosis. Plos Biol. 2021 Feb;19(2):e3001113 WB ; Human. PubMed:33626035 [IF=6.73] Yue, Huan. et al. LINC02154 promotes the proliferation and metastasis of hepatocellular carcinoma by enhancing SPC24 promoter activity and activating the PI3K-AKT signaling pathway. CELL ONCOL. 2022 May;:1-16 WB ; Human,Mouse. PubMed:35543858 [IF=6.691] Zhao, Boyuan. et al. Suspension state and shear stress enhance breast tumor cells EMT through YAP by microRNA-29b. 2021 Oct 07 WB ; Human. PubMed:34618275 [IF=6.63] Guo, Zhi-chen. et al. Porphyromonas gingivalis promotes the progression of oral squamous cell carcinoma by activating the neutrophil chemotaxis in the tumour microenvironment. CANCER IMMUNOL IMMUN. 2022 Dec;:1-17 WB ; Mouse. PubMed:36513851 [IF=6.217] Zhang J, et al. LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients.(2018) J. Exp. Clin. Cancer Res. Sep 12;37(1):225. IHC ; Human. PubMed:30208924 [IF=6.196] Cheng He. et al. Crosstalk of renal cell carcinoma cells and tumor-associated macrophages aggravates tumor progression by modulating muscleblind-like protein 2/B-cell lymphoma 2/beclin 1-mediated autophagy. CYTOTHERAPY. 2022 Oct;: WB, IHC, IF ; Mouse, Human. PubMed:36244911 [IF=6.023] Ling Xie. et al. Suppression of GOLM1 by EGCG through HGF/HGFR/AKT/GSK-3β/β-catenin/c-Myc signaling pathway inhibits cell migration of MDA-MB-231. Food Chem Toxicol. 2021 Nov;157:112574 WB ; human. PubMed:34536514 [IF=5.923] Nanami Nakamura. et al. Possible Action of Olaparib for Preventing Invasion of Oral Squamous Cell Carcinoma In Vitro and In Vivo. Int J Mol Sci. 2022 Jan;23(5):2527 WB ; Mouse. PubMed:10.3390/ijms23052527 [IF=5.572] Liu H et al. Anti-tubulin agent vinorelbine inhibits metastasis of cancer cells by regulating epithelial-mesenchymal transition. Eur J Med Chem. 2020 Aug 15;200:112332. WB ; Human. PubMed:32473523 [IF=5.572] Xu Yang. et al. Deoxynivalenol induces testicular ferroptosis by regulating the Nrf2/System Xc?/GPX4 axis. FOOD CHEM TOXICOL. 2023 May;175:113730 WB ; Mouse. PubMed:36925038 [IF=4.784] Zheng Wu. et al. FOXD3 suppresses epithelial–mesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22 WB ; human. PubMed:34551139 [IF=4.432] Botao Pan. et al. Vorinostat targets UBE2C to reverse epithelial-mesenchymal transition and control cervical cancer growth through the ubiquitination pathway. Eur J Pharmacol. 2021 Oct;908:174399 WB ; Human. PubMed:34331954 [IF=4.36] Yuhua Li. et al. Bolbostemma paniculatum (Maxim.) Franquet extract suppresses the development of colorectal cancer through downregulation of PI3K/Akt pathway. J Ethnopharmacol. 2022 Apr;287:114937 WB ; Human. PubMed:34958876 [IF=4.357] Fei et al. The number of polyploid giant cancer cells and epithelial-mesenchymal transition-related proteins are associated with invasion and metastasis in human breast cancer. (2015) J.Exp.Clin.Cancer.Res. 34:158 WB ; Human. PubMed:26702618 [IF=4.147] Francesca Salamanna. et al. Development and characterization of a novel human 3D model of bone metastasis from breast carcinoma in vitro cultured. Bone. 2021 Feb;143:115773 IHC ; Human. PubMed:33249322 [IF=4.12] Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543 WB ; Human. PubMed:27824118 [IF=4.101] Yu Guo. et al. RepSox effectively promotes the induced differentiation of sheep fibroblasts into adipocytes via the inhibition of the TGF?β1/Smad pathway. Int J Mol Med. 2021 Aug;48(2):1-13 WB ; Sheep. PubMed:34132357 [IF=4.101] Haixiang Ding. et al. Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med. 2022 Feb;49(2):1-11 WB ; Human. PubMed:34958109 [IF=4.068] Hao Cui. et al. Mena as a key enhancer factor of EMT to promote metastasis of human tongue squamous cell carcinoma. ORAL DIS. 2023 May;: WB ; Human. PubMed:37203597 [IF=3.98] Liu, Wen-bin, et al. "Aberrant methylation accounts for cell adhesion-related gene silencing during 3-methylcholanthrene and diethylnitrosamine induced multistep rat lung carcinogenesis associated with overexpression of DNA methyltransferases 1 and 3a." Toxicology and applied pharmacology 251.1 (2011): 70-78. IHC-P ; Rat. PubMed:21163286 [IF=3.493] Fangyu Chai. et al. Identification of SLC2A3 as a prognostic indicator correlated with the NF-κB/EMT axis and immune response in head and neck squamous cell carcinoma. CHANNELS. 2023;17(1):Article: 2208928 WB ; Human. PubMed:37134043 [IF=3.405] Wu Y et al. MFAP5 promotes basal-like breast cancer progression by activating the EMT program.Cell Biosci. 2019 Mar 7;9:24. WB ; Human. PubMed:30899449 [IF=3.391] Jasmine Ercoliet al. KRIT1 as a possible new player in melanoma aggressiveness. Arch Biochem Biophys . 2020 Sep 30;691:108483. WB ; Human. PubMed:32735866 [IF=3.26] Park, Honghyun, Doyun Kim, and Kuen Yong Lee. "Interaction‐tailored cell aggregates in alginate hydrogels for enhanced chondrogenic differentiation."Journal of Biomedical Materials Research Part A (2016). IHC-P ; Mouse. PubMed:27529335 [IF=3.111] Qi Rao. et al. Effects of dihydroartemisinin combined with cisplatin on proliferation, apoptosis and migration of HepG2 cells. ONCOL LETT. 2022 Aug;24(2):1-9 WB ; Human. PubMed:35782905 [IF=2.766] Ning et al. Vascular endothelial growth factor receptor-1 activation promotes migration and invasion of breast cancer cells through epithelial-mesenchymal transition. (2013) PLoS.One. 8:e65217 IHC-P ; Human. PubMed:23776453
研究領(lǐng)域	腫瘤細(xì)胞生物免疫學(xué) 激酶和磷酸酶細(xì)胞粘附分子
抗體來(lái)源	Rabbit
克隆類型	Polyclonal
交叉反應(yīng)	Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Horse)
產(chǎn)品應(yīng)用	WB=1:500-2000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	100kDa
細(xì)胞定位	細(xì)胞膜
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human N-cadherin: 701-800/905
亞型	IgG
純化方法	affinity purified by Protein A
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng)	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. Function: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. Subcellular Location: Cell membrane. Similarity: Contains 5 cadherin domains. SWISS: P19022 Gene ID: 1000 Database links: Entrez Gene: 1000 Human Entrez Gene: 281062 Cow Entrez Gene: 12558 Mouse Entrez Gene: 83501 Rat Omim: 114020 Human SwissProt: P19022 Human SwissProt: P15116 Mouse SwissProt: Q9Z1Y3 Rat Unigene: 464829 Human Unigene: 606106 Human Unigene: 257437 Mouse Unigene: 23200 Rat 細(xì)胞粘附蛋白（Call Adhesion Protein） N-鈣粘附分子屬于依賴鈣離子的細(xì)胞間糖蛋白粘附分子的一部分,主要是參與細(xì)胞間同源性粘附,調(diào)控和介導(dǎo)細(xì)胞間相互反應(yīng)。
產(chǎn)品圖片	Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 3: U251 (Human) Cell Lysate at 30 ug Lane 4: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-N-cadherin (bs-1172R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-N-cadherin (bs-1172R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kD Observed band size: 105/125 kD Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Cerebrum (Mouse) Lysate at 40 ug Lane 3: Testis (Rat) Lysate at 40 ug Lane 4: Cerebellum (Rat) Lysate at 40 ug Lane 5: Panc-1 (Human) Cell Lysate at 30 ug Lane 6: A549 (Human) Cell Lysate at 30 ug Lane 7: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 8: U251 (Human) Cell Lysate at 30 ug Primary: Anti-N-cadherin (bs-1172R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標(biāo)記二抗

	1、抗體溶解方法
	2、抗體修復(fù)方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問(wèn)題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問(wèn)題解答
	8、關(guān)于肽鏈的設(shè)計(jì)
	9、多肽的溶解與保存
	10、酶標(biāo)抗體效價(jià)測(cè)定程序

	AbBy Fluor 350 標(biāo)記
	AbBy Fluor 488 標(biāo)記
	AbBy Fluor 555 標(biāo)記
	AbBy Fluor 594 標(biāo)記
	AbBy Fluor 647 標(biāo)記
	AP 標(biāo)記
	APC 標(biāo)記
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	Cy5.5 標(biāo)記
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	FITC 標(biāo)記
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	HRP 標(biāo)記
	PE 標(biāo)記
	PE-Cy3 標(biāo)記
	PE-CY5 標(biāo)記
	PE-CY5.5 標(biāo)記
	PE-CY7 標(biāo)記
	RBITC 標(biāo)記

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