| 產(chǎn)品編號 | bsm-0917M |
| 英文名稱 | Mouse Anti-BrdU(Proliferation Marker) antibody |
| 中文名稱 | 5-溴脫氧尿嘧啶核苷（增殖標志物）單克隆抗體 |
| 別 名 | Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine. |
 |
Specific References (8) | bsm-0917M has been referenced in 8 publications.
|
| 產(chǎn)品類型 | 小分子抗體 |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 發(fā)育生物學(xué) 染色質(zhì)和核信號 神經(jīng)生物學(xué) 藥物及化合物 細胞類型標志物 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 1A7 |
| 交叉反應(yīng) | Species independent |
| 產(chǎn)品應(yīng)用 | ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | .3071kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Brdu |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Proliferation Marker Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood (Campana et al., 1988), tissues (Schutte et al., 1987; Hayashi, et al., 1988), tumors (Hoshino et al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling indices (Greipp et al., 1985). Subcellular Location: Nuclear. SWISS: N/A CAS: 59-14-3 增殖標志物 BrdU（溴化脫氧尿嘧啶核苷）可以在體內(nèi)和體外摻入到處于S期的細胞所合成的DNA鏈中。此抗體可以與摻入到任何種屬細胞中的BrdU反應(yīng)，與碘脫氧尿嘧啶有交叉反應(yīng)，標記摻有BrdU的S期細胞，主要用于研究各種不同的因素對正常/腫瘤組織的細胞增殖及動力學(xué)的研究。 用FITC標記的抗BrdU IgG可用于流式細胞術(shù)定量檢測增值細胞。 |
| 產(chǎn)品圖片 |
BrdU treated (10uM for 24 hours) Hela cells,4% Paraformaldehyde-fixed MCF-7 (H) cell; 2M HCL at r.t. for 20 min; Antibody incubation with (BrdU ) monoclonal Antibody, unconjugated (bsm-0917M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei.
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
