| 產(chǎn)品編號(hào) | bs-2514R |
| 英文名稱 | Rabbit Anti-CCR5 antibody |
| 中文名稱 | 細(xì)胞表面趨化因子受體5抗體 |
| 別 名 | C-C chemokine receptor 5; CCR5; CC-CKR-5;CCR-5; CCCKR5; CD195; CD 195; CKR-5; CKR5; CMKBR5; FLJ78003; IDDM22; AM4-7; C C chemokine receptor type 5; C-C motif chemokine receptor 5 A159A; CC Chemokine Receptor Type 5; CCR5 chemokine (C C motif) receptor 5; Chemokine receptor CCR5; CHEMR13; HIV 1 Fusion Coreceptor; HIV1 fusion coreceptor; IDDM22; MIP-1 alpha receptor; CCR5_HUMAN. |
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Specific References (6) | bs-2514R has been referenced in 6 publications.
[IF=10.317] Jianxiong Xu. et al. Nanoparticles retard immune cells recruitment in vivo by inhibiting chemokine expression. Biomaterials. 2021 Jan;265:120392 WB ; Human.
[IF=5.793] Nicolas Pelisch. et al. CCL3 contributes to secondary damage after spinal cord injury. J Neuroinflamm. 2020 Dec;17(1):1-16 WB ; Mouse.
[IF=5.719] Subhra Mandal. et al. Targeted Immuno-Antiretroviral to Promote Dual Protection against HIV: A Proof-of-Concept Study. NANOMATERIALS-BASEL. 2022 Jan;12(11):1942 FC ; Human.
[IF=5.714] Tianming Li. et al. IL-17D affects the chemokines and chemokine receptors of intestinal epithelial cells under hyperoxia. INT IMMUNOPHARMACOL. 2022 Dec;113:109386 IHC ; Rat, Human.
[IF=4.321] Lisa Detering. et al. CC Chemokine Receptor 5 Targeted Nanoparticles Imaging the Progression and Regression of Atherosclerosis Using Positron Emission Tomography/Computed Tomography. Mol Pharmaceut. 2021;18(3):1386–1396 IF,IHC ; Mouse, Human.
[IF=3.73] Ochoa-Callejero, Laura, et al. "Maraviroc, a CCR5 Antagonist, Prevents Development of Hepatocellular Carcinoma in a Mouse Model." PloS one 8.1 (2013): e53992. IHC-P ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 細(xì)胞膜受體 細(xì)胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CCR-5: 201-300/352 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]. Function: Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. Subunit: Interacts with PRAF2. Interacts with HIV-1 surface protein gp120. Efficient ligand binding to CCL3/MIP-1alpha and CCL4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on Ser-6 is required for efficient binding of CCL4. Interacts with ADRBK1. Interacts with ARRB1 and ARRB2. Interacts with human cytomegalovirus/HHV-5 protein UL78. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P51681 Gene ID: 1234 Database links: Entrez Gene: 1234 Human Entrez Gene: 12774 Mouse Omim: 601373 Human SwissProt: P51681 Human SwissProt: P51682 Mouse Unigene: 450802 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human THP-1 cell lysates
Lane 2: Human U937 cell lysates
Primary: Anti-CCR-5 (bs-2514R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 43 kD
Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)) . Primary Antibody:Rabbit Anti-CCR5 antibody(bs-2514R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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