| 產(chǎn)品編號 | bs-2168R |
| 英文名稱 | Rabbit Anti-ADAR1 antibody |
| 中文名稱 | 雙鏈RNA腺苷酸脫氨基酶（C端）抗體 |
| 別 名 | 136kDa double stranded RNA binding protein; Adar 1; ADAR; Adar1; Adenosine deaminase RNA specific 1; Adenosine deaminase RNA specific; Adenosine deaminase that act on RNA; AV242451; Double stranded RNA specific adenosine deaminase; Double-stranded RNA-specific editase Adar; Drada; Dsh; Dsrad; dsRNA adenosine deaminase; EC 3.5.4.-; G1P1; IFI 4; IFI4; Ifi4 protein; Interferon induced protein 4; Interferon inducible protein 4; K88dsrbp; mZaADAR; p136; Pre-mRNA adenosine deaminase; RNA adenosine deaminase 1; RNA-editing deaminase 1; RNA-editing enzyme 1. |
 |
Specific References (2) | bs-2168R has been referenced in 2 publications.
[IF=6.543] Zheng Ping. et al. circHtra1/miR-3960/GRB10 Axis Promotes Neuronal Loss and Immune Deficiency in Traumatic Brain Injury. OXID MED CELL LONGEV. 2022;2022:3522492 WB ; Human.
[IF=6.481] Li Jia. et al. Integrated analysis of RNA methylation regulators crosstalk and immune infiltration for predictive and personalized therapy of diabetic nephropathy. HUM GENOMICS. 2023 Dec;17(1):1-19 WB,IF ; Mouse,Human.
|
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 細(xì)胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 135kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DRADA: 1001-1226/1226 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010] Function: Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression. Subunit: Homodimer. Isoform 1 interacts with ILF2/NF45 and ILF3/NF90. Subcellular Location: Cytoplasm. Nucleus, nucleolus. Isoform 1: Cytoplasm. Note=Found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5: Nucleus, nucleolus. Tissue Specificity: Ubiquitously expressed, highest levels were found in brain and lung. Post-translational modifications: Sumoylation reduces RNA-editing activity. DISEASE: Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet. Similarity: Contains 1 A to I editase domain. Contains 2 DRADA repeats. Contains 3 DRBM (double-stranded RNA-binding) domains. SWISS: P55265 Gene ID: 103 Database links: Entrez Gene: 103 Human Entrez Gene: 56417 Mouse Omim: 146920 Human SwissProt: P55265 Human SwissProt: Q99MU3 Mouse Unigene: 12341 Human Unigene: 679967 Human Unigene: 316628 Mouse |
| 產(chǎn)品圖片 |
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-ADAR1 (bs-2168R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 135 kD
Observed band size: 110 kD
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-ADAR1 (bs-2168R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 135 kD
Observed band size: 110 kD
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-ADAR1 (bs-2168R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 135 kD
Observed band size: 110 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADAR1) Polyclonal Antibody, Unconjugated (bs-2168R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
|
