| 產(chǎn)品編號(hào) | bs-2887R |
| 英文名稱 | Rabbit Anti-CYP2C9 antibody |
| 中文名稱 | 細(xì)胞色素P450 2C9抗體 |
| 別 名 | Cytochrome P450 2C9; The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme CPC9; CYP2C; CYP2C10; CYP2C9; Cytochrome p4502C9; P450 MP; P450 PB 1; P450 PB1; P450IIC9; P450MP; S mephenytoin 4 hydroxylase; CP2C9_HUMAN. |
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Specific References (4) | bs-2887R has been referenced in 4 publications.
[IF=5.324] Wang Shanshan. et al. Gut microbiota and host cytochrome P450 characteristics in the pseudo germ-free model: co-contributors to a diverse metabolic landscape. GUT PATHOG. 2023 Dec;15(1):1-14 WB ; Rat.
[IF=5.275] Kai Wang. et al. Drug-drug interactions induced by Linderane based on mechanism-based inactivation of CYP2C9 and the molecular mechanisms. Bioorg Chem. 2022 Jan;118:105478 WB ; Mouse.
[IF=5.17] Zhang et al. Hyperhomocysteinemia results from and promotes hepatocellular carcinoma via CYP450 metabolism by CYP2J2 DNA methylation. (2017) Oncotarge. 8:15377-15392 WB ; Human.
[IF=4.15] Jiang, Zhihui, et al. "Development of an IgY Antibody-Based Immunoassay for the Screening of the CYP2E1 Inhibitor/Enhancer from Herbal Medicines." Frontiers in Pharmacology 7 (2016): 502. WB ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 生長(zhǎng)因子和激素 新陳代謝 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:200-800,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CYP2C9: 351-450/490 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq, Jul 2008] Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan. Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Similarity: Belongs to the cytochrome P450 family. SWISS: P11712 Gene ID: 1559 Database links: Entrez Gene: 1559 Human Omim: 601130 Human SwissProt: P11712 Human Unigene: 282624 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Stomach tissue lysates
Primary: Anti-CYP2C9 (bs-2887R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 52 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP2C9) Polyclonal Antibody, Unconjugated (bs-2887R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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