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Rabbit Anti-Insulin  antibody (bs-0855R)  
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產(chǎn)品編號(hào) bs-0855R
英文名稱(chēng) Rabbit Anti-Insulin  antibody
中文名稱(chēng) 胰島素抗體
別    名 ILPR; INS; Insulin A chain; Insulin B chain; Insulin A chain; Insulin precursor; IRDN; Proinsulin; Proinsulin precursor; IDDM2; INS_HUMAN; MODY10.  
Specific References  (1)     |     bs-0855R has been referenced in 1 publications.
[IF=5.705] Sun HD et al. Electrochemical sandwich immunoassay for insulin detection based on the use of gold nanoparticle-modified MoS 2 nanosheets and the hybridization chain reaction. Microchimica Acta,2018 186(1).  sandwich immunoreaction ;  
研究領(lǐng)域 腫瘤  心血管  細(xì)胞生物  神經(jīng)生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  生長(zhǎng)因子和激素  糖尿病  內(nèi)分泌病  新陳代謝  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant human insulin 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Insulin is a pancreatic hormone that regulates glucose and is involved in the synthesis of protein and fat. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds.Belongs to the insulin family. The insulin-link growth factors, IGF-I and IGF-II (also desinated somatomedin C and multiplication stimulating activator, respectvely), share approximatly 76% sequence identity and are 50% related to pro-insulin.IGF-I and IGF-II are nonglycosylated, single chain proteins of 70 and 76 amino acids in length, respectivelly. IGF-I functions as an autocrine regulator of growth in vaious, whereas the function of IGF-II is less well defined.

Function:
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Subunit:
Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Subcellular Location:
Secreted.

DISEASE:
Hyperproinsulinemia, familial (FHPRI) [MIM:176730]: An autosomal dominant condition characterized by elevated levels of serum proinsulin-like material. Note=The disease is caused by mutations affecting the gene represented in this entry.
Diabetes mellitus, insulin-dependent, 2 (IDDM2) [MIM:125852]: A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Note=The disease is caused by mutations affecting the gene represented in this entry.
Diabetes mellitus, permanent neonatal (PNDM) [MIM:606176]: A rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy. Note=The disease is caused by mutations affecting the gene represented in this entry.
Maturity-onset diabetes of the young 10 (MODY10) [MIM:613370]: A form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the insulin family.

SWISS:
P01308

Gene ID:
3630

Database links:

Entrez Gene: 3630 Human

Entrez Gene: 280829 Cow

Entrez Gene: 16333 Mouse

Entrez Gene: 16334 Mouse

Entrez Gene: 24505 Rat

Entrez Gene: 397415 Pig

SwissProt: P01308 Human

SwissProt: P01325 Mouse

SwissProt: P01322 Rat

SwissProt: P01315 Pig




胰島素(Isulin)胰島素抗體是胰島細(xì)胞分泌得一種激素,可以減底血糖濃度。此抗體和人胰島素反應(yīng),并與大多數(shù)哺乳類(lèi)動(dòng)物的胰島素有交叉反應(yīng),主要用于胰島細(xì)胞瘤的功能性研究。
產(chǎn)品圖片
Sample: Lane 1: Recombinant human Insulin protein, N-Trx-His(bs-42257P) Primary: Anti-Insulin (bs-0855R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kDa Observed band size: 26 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Insulin) Polyclonal Antibody, Unconjugated (bs-0855R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Insulin) Polyclonal Antibody, Unconjugated (bs-0855R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Insulin) Polyclonal Antibody, Unconjugated (bs-0855R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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