| 產(chǎn)品編號 | bs-3699R |
| 英文名稱 | Rabbit Anti-GPR15 antibody |
| 中文名稱 | G蛋白偶聯(lián)受體15抗體 |
| 別 名 | GPCR GPR15; BOB; G protein coupled receptor 15; G protein coupled receptor GPR15; GPR15_HUMAN; G-protein coupled receptor 15; Brother of Bonzo. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) G蛋白偶聯(lián)受體 細胞表面分子 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:200-800
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR15: 51-150/360 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a G protein-coupled receptor that acts as a chemokine receptor for human immunodeficiency virus type 1 and 2. The encoded protein localizes to the cell membrane. [provided by RefSeq, Nov 2012] Function: Probable chemokine receptor. Alternative coreceptor with CD4 for HIV-1 infection. Subcellular Location: Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P49685 Gene ID: 2838 Database links: Entrez Gene: 2838 Human Entrez Gene: 71223 Mouse Omim: 601166 Human SwissProt: P49685 Human SwissProt: Q0VDU3 Mouse Unigene: 563128 Human Unigene: 359970 Mouse Unigene: 426544 Mouse G蛋白偶聯(lián)受體是一種細胞表面受體，能將細胞外的分子信號傳遞至細胞內(nèi)激起細胞反應(yīng)，對多種細胞行起調(diào)控作用。G蛋白偶聯(lián)受體（GPCR）參與了機體內(nèi)多種生理過程的調(diào)節(jié)，是目前治療多種人類疾病包括代謝性疾病的藥物靶點。 |
| 產(chǎn)品圖片 |
Tissue/cell: Mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR15 Polyclonal Antibody, Unconjugated(bs-3699R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat stomach tissue; Frozen section;
Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GPR15 Polyclonal Antibody, Unconjugated(bs-3699R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
