| 產(chǎn)品編號 | bs-2333R |
| 英文名稱 | Rabbit Anti-NPRC antibody |
| 中文名稱 | 利鈉肽受體C抗體 |
| 別 名 | ANP C; ANPC; ANPRC; Atrial natriuretic peptide C type receptor; Atrionatriuretic peptide receptor C; Natriuretic peptide receptor C/guanylate cyclase C; NPR 3; NPR C; NPR3; NPRC; ANPRC_HUMAN. |
 |
Specific References (1) | bs-2333R has been referenced in 1 publications.
[IF=4.315] Shuo Li. et al. NPR3, transcriptionally regulated by POU2F1, inhibits osteosarcoma cell growth through blocking the PI3K/AKT pathway. Cell Signal. 2021 Oct;86:110074 IHC ; Mouse.
|
| 研究領(lǐng)域 | 心血管 信號轉(zhuǎn)導 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Cow,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NPR-C: 301-400/541 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Natriuretic peptide receptor C does not exhibit guanylate cyclase activity. There seem to be at least three ANP receptors: two with guanylate cyclase activity (ANPA and ANPB) and one (ANPC) which is probably responsible for the clearance of ANP from the circulation without a role in signal transduction. Function: Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity. Subunit: Homodimer; disulfide-linked. Dimers can also be formed through the C-terminal cysteine of isoform 2. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Belongs to the ANF receptor family. SWISS: P17342 Gene ID: 4883 Database links: Entrez Gene: 4883 Human Entrez Gene: 18162 Mouse Omim: 108962 Human SwissProt: P17342 Human SwissProt: P70180 Mouse Unigene: 237028 Human Unigene: 619466 Human Unigene: 25259 Mouse Unigene: 11297 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human A549 cell lysates
Lane 3: Human U87MG cell lysates
Primary: Anti-NPRC (bs-2333R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 62 kDa
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NPRC) Polyclonal Antibody, Unconjugated (bs-2333R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
