| 產(chǎn)品編號(hào) | bs-3929R |
| 英文名稱 | Rabbit Anti-ACLY antibody |
| 中文名稱 | 三磷酸腺檸檬酸裂解酶抗體 |
| 別 名 | ATP citrate lyase; ACL; ATP citrate (pro-S) lyase; ATP citrate synthase; Citrate cleavage enzyme; EC 2.3.3.8; A730098H14RIK; ATP CITRATE LYASE; ATPCL; AW538652; Cce; CITRATE LYASE; CLATP; MGC124629; ACLY_HUMAN. |
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Specific References (1) | bs-3929R has been referenced in 1 publications.
[IF=6.78] Zhe Jiang. et al. RSL1D1 modulates cell senescence and proliferation via regulation of PPARγ mRNA stability. LIFE SCI. 2022 Aug;:120848 WB ; Human.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 激酶和磷酸酶 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 122kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ACLY: 951-1101/1101 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq] Function: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Subunit: Homotetramer. Subcellular Location: Cytoplasm. Post-translational modifications: ISGylated. Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2. Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site. Similarity: In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family. In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family. SWISS: P53396 Gene ID: 47 Database links: Entrez Gene: 47 Human Entrez Gene: 104112 Mouse Omim: 108728 Human SwissProt: P53396 Human SwissProt: Q91V92 Mouse Unigene: 387567 Human Unigene: 282039 Mouse Unigene: 29771 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human A549 cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human 293T cell lysates
Primary: Anti-ACLY (bs-3929R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 122 kDa
Observed band size: 115 kDa
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