| 產(chǎn)品編號 | bs-5085R |
| 英文名稱 | Rabbit Anti-MVD antibody |
| 中文名稱 | 甲羥戊酸脫羧酶抗體 |
| 別 名 | Diphosphomevalonate decarboxylase; FP17780; MDDase; Mevalonate (diphospho) decarboxylase; Mevalonate (diphospho)decarboxylase; Mevalonate pyrophosphate decarboxylase; MPD; Mvd; MVD1_HUMAN． |
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Specific References (2) | bs-5085R has been referenced in 2 publications.
[IF=2.629] Li Yanchu. et al. Effects of a Chlorogenic Acid-Containing Herbal Medicine (LASNB) on Colon Cancer. Evid-Based Compl Alt. 2021;2021:9923467 IHC ; Mouse.
[IF=1.813] Li Y et al. Study on the Anticancer Effect of an Astragaloside- and Chlorogenic Acid-Containing Herbal Medicine (RLT-03) in Breast Cancer. Evid Based Complement Alternat Med. 2020 Jun 10;2020:1515081. IHC-P ; Human.
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| 研究領(lǐng)域 | 腫瘤 心血管 細胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 脂蛋白 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MVD: 201-300/400 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The enzyme mevalonate pyrophosphate decarboxylase (MVD) catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate in one of the early steps in cholesterol biosynthesis. It decarboxylates and dehydrates its substrate while hydrolyzing ATP. Function: Performs the first committed step in the biosynthesis of isoprenes. Subunit: Homodimer. Tissue Specificity: Expressed in heart, skeletal muscle, lung,liver, brain, pancreas, kidney and placenta. Similarity: Belongs to the diphosphomevalonate decarboxylase family. SWISS: P53602 Gene ID: 4597 Database links: Entrez Gene: 4597 Human Entrez Gene: 192156 Mouse Omim: 603236 Human SwissProt: P53602 Human SwissProt: Q99JF5 Mouse Unigene: 252457 Human Unigene: 28146 Mouse Unigene: 10288 Rat |
| 產(chǎn)品圖片 |
Sample:
H9C2(Rat) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-MVD (bs-5085R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MVD) Polyclonal Antibody, Unconjugated (bs-5085R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
