| 產(chǎn)品編號 | bs-2381R |
| 英文名稱 | Rabbit Anti-SRP68 antibody |
| 中文名稱 | 信號識別顆?？贵w |
| 別 名 | Signal recognition particle 68 kDa; Signal recognition particle subunit SRP68; Signal recognition particle 68 kDa protein; SRP 68; Srp68; SRP68_HUMAN. |
| 研究領(lǐng)域 | 免疫學(xué) 染色質(zhì)和核信號 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 71kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SRP68: 401-500/627 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP68 binds the 7S RNA, SRP72 binds to this complex subsequently. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function. Function: Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP68 binds the 7S RNA, SRP72 binds to this complex subsequently. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function. Subunit: Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. Subcellular Location: Cytoplasm. Nucleus, nucleolus. Similarity: Belongs to the SRP68 family. SWISS: Q9UHB9 Gene ID: 6730 Database links: Entrez Gene: 6730 Human Entrez Gene: 217337 Mouse Omim: 604858 Human SwissProt: Q9UHB9 Human SwissProt: Q8BMA6 Mouse Unigene: 514495 Human Unigene: 29655 Mouse Unigene: 68 Rat 信號識別顆粒(Signalrecognitionparticle,SRP)是介導(dǎo)蛋白識別轉(zhuǎn)運其中普遍存在的機制之一,細胞內(nèi)蛋白質(zhì)的定向轉(zhuǎn)移是蛋白質(zhì)質(zhì)量數(shù)量控制體系的一個重要環(huán)節(jié),SRP對參與這一過程的功能基因和蛋白,尤其是蛋白的靶向輸送機制的研究對揭示細胞的分化、代謝和病變具有重大的理論意義. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SRP68) Polyclonal Antibody, Unconjugated (bs-2381R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
