| 產(chǎn)品編號 | bs-2973R |
| 英文名稱 | Rabbit Anti-SMAD1 + SMAD5 antibody |
| 中文名稱 | 細胞信號轉(zhuǎn)導分子SMAD1 + SMAD5抗體 |
| 別 名 | Dwfc; hSmad 5; hSmad5; JV5 1; JV5-1; MAD homolog 5; MAD mothers against decapentaplegic homolog 5; MAD, mothers against decapentaplegic homolog 5; MADH 5; MADH5; Mothers against decapentaplegic homolog 5; Mothers against DPP homolog 5; MusMLP; SMA and MAD related protein 5; SMAD 5; SMAD family member 5; SMAD mothers against DPP homolog 5; Smad5; SMAD5_HUMAN. |
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Specific References (1) | bs-2973R has been referenced in 1 publications.
[IF=1.663] Yu M et al. BMP4?mutations?in?tooth?agenesis?and?low?bone?mass. Arch Oral Biol.?2019 Jul;103:40-46. WB ; Human.
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| 研究領域 | 細胞生物 染色質(zhì)和核信號 信號轉(zhuǎn)導 干細胞 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rabbit (predicted: Rat,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:200-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SMAD5: 361-465/465 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] Function: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). Subcellular Location: Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. Similarity: Belongs to the dwarfin/SMAD family. Contains 1 MH1 (MAD homology 1) domain. Contains 1 MH2 (MAD homology 2) domain. SWISS: Q99717 Gene ID: 4090 Database links: Entrez Gene: 4090 Human Entrez Gene: 17129 Mouse Omim: 603110 Human SwissProt: Q99717 Human SwissProt: P97454 Mouse Unigene: 167700 Human Unigene: 272920 Mouse Unigene: 146857 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-SMAD1 + SMAD5 (bs-2973R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 55 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMAD1 + SMAD5) Polyclonal Antibody, Unconjugated (bs-2973R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SMAD1 + SMAD5) polyclonal Antibody, Unconjugated (bs-2973R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
