| 產(chǎn)品編號 | bs-6208R |
| 英文名稱 | Rabbit Anti-TMED4 antibody |
| 中文名稱 | 跨膜蛋白TMED4抗體 |
| 別 名 | HNLF; Transmembrane emp24 domain containing protein 4; TMED4_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TMED4: 41-140/227 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
TMED4 is a putative NF-kB activating protein belonging to the EMP24/GP25L family, and contains 1 GOLD domain. It is also known as Transmembrane emp24 protein transport domain containing 4. Function: Involved in vesicular protein trafficking, mainly in the early secretory pathway. targeting. Involved in the maintenance of the Golgi apparatus. Appears to play a role in the biosynthesis of secreted cargo including processing. Involved in endoplasmic reticulum stress response. May play a role in the regulation of heat-shock response and apoptosis Subcellular Location: Endoplasmic reticulum membrane; Single-pass type I membrane protein Similarity: Belongs to the EMP24/GP25L family. Contains 1 GOLD domain. SWISS: Q7Z7H5 Gene ID: 222068 Database links: Entrez Gene: 222068 Human Entrez Gene: 103694 Mouse Omim: 612038 Human SwissProt: Q7Z7H5 Human SwissProt: Q8R1V4 Mouse Unigene: 598832 Human Unigene: 715273 Human Unigene: 254495 Mouse |
| 產(chǎn)品圖片 |
Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-TMED4 (bs-6208R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 23 kD
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMED4) Polyclonal Antibody, Unconjugated (bs-6208R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
