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Rabbit Anti-LECT1  antibody (bs-7516R)  
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產(chǎn)品編號(hào) bs-7516R
英文名稱 Rabbit Anti-LECT1  antibody
中文名稱 白細(xì)胞衍生化學(xué)吸引素抗體
別    名 Chondromodulin I; BRICD3; BRICHOS domain containing 3; CHM I; CHM1; chondromodulin; Chondrosurfactant protein; LECT 1; Leukocyte cell derived chemotaxin 1; LECT1_HUMAN.  
Specific References  (1)     |     bs-7516R has been referenced in 1 publications.
[IF=2.061] Kumagai Y et al. Chondromodulin-1 and vascular endothelial growth factor-A expression in esophageal squamous cell carcinoma: accelerator and brake theory for angiogenesis at the early stage of cancer progression. Esophagus. 2019 Oct 8.  IHC-P ;  Human.  
研究領(lǐng)域 腫瘤  心血管  細(xì)胞生物  免疫學(xué)  細(xì)胞周期蛋白  細(xì)胞骨架  細(xì)胞外基質(zhì)  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Dog,GuineaPig)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 14kDa
細(xì)胞定位 細(xì)胞膜 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human LECT1: 231-334/334 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The LECT1 gene encodes a glycosylated transmembrane protein which is cleaved to form a mature, secreted protein. The N terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein, although no biological activity has yet been defined for it. The C terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell derived chondromodulin 1 or chemotaxin 1. The mature protein inhibits angiogenesis and promotes chondrocyte growth. This gene is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development. Alternative splicing results in multiple transcript variants encoding different isoforms.

Function:
Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development.

Subcellular Location:
Chondromodulin-1: Secreted. Note=Accumulated in the inter-territorial matrix of cartilage. Chondrosurfactant protein: Endomembrane system; Single-pass membrane protein (Potential).

Tissue Specificity:
Cartilage specific. Weakly expressed in chondrosarcoma.

Similarity:
Belongs to the chondromodulin-1 family.
Contains 1 BRICHOS domain.

SWISS:
O75829

Gene ID:
11061

Database links:

UniProtKB/Swiss-Prot: O75829.1



產(chǎn)品圖片
Sample: Lane 1: Rat Eye tissue lysates Lane 2: Rat Cartilage tissue lysates Primary: Anti-LECT1 (bs-7516R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 45 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LECT1) Polyclonal Antibody, Unconjugated (bs-7516R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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