| 產品編號 | bs-2054R |
| 英文名稱 | Rabbit Anti-Nkx2.5 antibody |
| 中文名稱 | 心臟特異性同源盒轉錄因子NKX2.5抗體 |
| 別 名 | Cardiac-specific homeobox 1; Cardiac-specific homeobox 1; Cardiac-specific homeobox; CHNG5; CSX; CSX1; Homeobox protein CSX; Homeobox protein NK-2 homolog E; NK2 transcription factor related, locus 5 (Drosophila); Nkx2-5; NKX2.5; Nkx2E; NKX4-1; Tinman paralog. |
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Specific References (2) | bs-2054R has been referenced in 2 publications.
[IF=2.649] Kim CW et al. Effects of cigarette smoke components on myocardial differentiation of mouse embryonic stem cells. Environ Toxicol. 2019 Sep 10. WB ; Mouse.
[IF=1.86] Ruan, Zhong-Bao, et al. "Inhibitor of p53–p21 pathway induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyogenic cells." Cytotechnology (2015): 1-9. IF(ICC) ; Human.
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| 研究領域 | 心血管 細胞生物 發(fā)育生物學 神經生物學 干細胞 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Dog) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nkx2.5: 85-180/324 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
In humans, mutations of the gene encoding the homeobox transcription factor Nkx2.5 result in electrical conduction defects and morphological abnormalities of the heart (Dupays et al., 2005). In the heart, Nkx2.5 is expressed in both the myocardium and the endocardium. Differentiation of embryonic stem cells to Nkx2.5-positive cardiomyocytes is facilitated by Wnt11. Subcellular Location: Nuclear SWISS: P42582 Gene ID: 1482 Database links: Clone ID: 396073 Chicken Entrez Gene: 1482 Human Entrez Gene: 18091 Mouse Omim: 600584 Human SwissProt: Q90788 Chicken SwissProt: P52952 Human SwissProt: P42582 Mouse Unigene: 638 Chicken Unigene: 54473 Human Unigene: 41974 Mouse Unigene: 6179 Rat |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nkx2.5) Polyclonal Antibody, Unconjugated (bs-2054R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: Rat heart; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nkx2.5/Cardiac-specific homeobox 1 Polyclonal Antibody, Unconjugated (bs-2054R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
