| 產(chǎn)品編號(hào) | bs-7103R |
| 英文名稱 | Rabbit Anti-THAP1 antibody |
| 中文名稱 | 核凋亡因子THAP1抗體 |
| 別 名 | Nuclear proapoptotic factor; THAP 1; THAP domain containing apoptosis associated protein 1; THAP domain containing protein 1; THAP domain protein 1; THAP1_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 染色質(zhì)和核信號(hào) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 細(xì)胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Sheep,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human THAP1: 121-213/213 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
THAP1 contains a THAP domain, a zinc-dependent DNA-binding domain. It colocalizes with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies and is a pro-apoptopic protein that potentiates both serum-withdrawal and TNF-induced apoptosis. It is a physiologic regulator of endothelial cell proliferation and cell-cycle progression, two essential processes for angiogenesis. Function: DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes, including RRM1. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. May also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. Subunit: Interacts with PAWR. Component of a THAP1/THAP3-HCFC1-OGT complex that contains, either THAP1 or THAP3, HCFC1 and OGT. Interacts with OGT. Interacts (via the HBM) with HCFC1 (via the Kelch-repeat domain); the interaction recruits HCFC1 to the RRM1 promoter. Subcellular Location: Nucleus, nucleoplasm. Nucleus, PML body. Tissue Specificity: Highly expressed in heart, skeletal muscle, kidney and liver. Weaker expression in brain and placenta. DISEASE: Defects in THAP1 are the cause of dystonia type 6 (DYT6) [MIM:602629]. DYT6 is a primary torsion dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Dystonia type 6 is characterized by onset in early adulthood, cranial or cervical involvement in about half of the cases, and frequent progression to involve multiple body regions. Similarity: Belongs to the THAP1 family. Contains 1 THAP-type zinc finger. SWISS: Q9NVV9 Gene ID: 55145 Database links: Entrez Gene: 55145 Human Omim: 609520 Human SwissProt: Q9NVV9 Human Unigene: 7432 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Mouse Liver tissue lysates
Lane 4: Mouse Thymus tissue lysates
Lane 5: Rat Heart tissue lysates
Lane 6: Rat Muscle tissue lysates
Lane 7: Rat Kidney tissue lysates
Lane 8: Rat Liver tissue lysates
Lane 9: Rat Thymus tissue lysates
Lane 10: Human Molt-4 cell lysates
Lane 11: Human Huvec cell lysates
Lane 12: Human U251 cell lysates
Primary: Anti-THAP1 (bs-7103R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 27 kD
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