| 產(chǎn)品編號 | bs-9141R |
| 英文名稱 | Rabbit Anti-ZNRF3 antibody |
| 中文名稱 | 鋅指蛋白3/環(huán)指蛋白3抗體 |
| 別 名 | Novel C3HC4 type Zinc finger (ring finger); RING finger protein 203; RNF203; Zinc and ring finger 3; Zinc/RING finger protein 3; Znrf3; ZNRF3_HUMAN. |
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Specific References (2) | bs-9141R has been referenced in 2 publications.
[IF=12.121] Sarina Harshuk-Shabso. et al. Fgf and Wnt signaling interaction in the mesenchymal niche regulates the murine hair cycle clock. Nat Commun. 2020 Oct;11(1):1-14 IF,IHC ; Mouse.
[IF=4.573] Federico Pecori. et al. Mucin-type O-glycosylation controls pluripotency in mouse embryonic stem cells via Wnt receptor endocytosis. J Cell Sci. 2020 Oct;133(20) IHC ; Mouse.
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| 研究領(lǐng)域 | 細胞生物 免疫學 神經(jīng)生物學 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 95kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNRF3: 101-200/936 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). ZNRF3 (Zinc/RING finger protein 3), also known as RNF203 (RING finger protein 203), is a 936 amino acid single pass transmembrane protein that contains one RING-type zinc finger. Related zinc/RING finger proteins, such as ZNRF1 and ZNRF2, are E3 ubiquitin-protein ligases that are thought to be involved in the establishment and maintenance of neuronal transmission and plasticity, therefore it is likely that ZNRF3 may function in a similar manner. Function: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. Subunit: Interacts with LRP6, FZD4, FZD5, FZD6 and FZD8. Interacts with RSPO1; interaction promotes indirect interaction with LGR4 and membrane clearance of ZNRF3. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Similarity: Belongs to the ZNRF3 family. Contains 1 RING-type zinc finger. SWISS: Q9ULT6 Gene ID: 84133 Database links: Entrez Gene: 84133 Human Omim: 612062 Human SwissProt: Q9ULT6 Human Unigene: 655242 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HepG2 cell Lysates
Lane 2: Human A549 cell Lysates
Lane 3: Human U-2 OS cell Lysates
Primary: Anti-ZNRF3 (bs-9141R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95kDa
Observed band size: 120kDa
25 ug total protein per lane of various lysates (see on figure) probed with ZNRF3 polyclonal antibody, unconjugated (bs-9141R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNRF3) Polyclonal Antibody, Unconjugated (bs-9141R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
