| 產(chǎn)品編號 | bs-4902R |
| 英文名稱 | Rabbit Anti-Calponin 1 + 2 + 3 antibody |
| 中文名稱 | 鈣結(jié)合蛋白Calponin抗體 |
| 別 名 | smooth muscle; Basic calponin; Calponin 1; Calponin 2; Calponin 3; Calponin1; Calponin2; Calponin3; Calponin-1; Calponin-2; Calponin-3; Calponin 1 basic smooth muscle; Calponin H1; Calponin H1 smooth muscle; Calponins basic; CNN 1; Cnn1; CNN2_HUMAN; Sm Calp; SMCC. |
| 研究領(lǐng)域 | 心血管 細胞生物 信號轉(zhuǎn)導(dǎo) 干細胞 結(jié)合蛋白 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細胞定位 | 細胞漿 細胞膜 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Calponin 1/2/3: 101-200/309 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Calponin regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an Actin- and Tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-Actin and inhibition of the Actin-activated MgATPase activity of phosphorylated myosin. Both properties are lost following phosphorylation (primarily at Serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin) and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain. Function: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Tissue Specificity: Heart and smooth muscle. Similarity: Belongs to the calponin family. Contains 3 calponin-like repeats. Contains 1 CH (calponin-homology) domain. SWISS: Q99439 Gene ID: 1265 Database links: Entrez Gene: 1265 Human Omim: 602373 Human SwissProt: Q99439 Human Unigene: 651512 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Calponin 1 + 2 + 3) Polyclonal Antibody, Unconjugated (bs-4902R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Positive control: H9C2(2% Paraformaldehyde-fixed )
Isotype Control Antibody: Rabbit IgG;
Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA
Secondary Antibody: Goat anti-rabbit IgG-FITC;
Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody: rabbit Anti-Calponin 1 + 2 + 3 (bs-4902R);
Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
