| 產(chǎn)品編號 | bs-9880R |
| 英文名稱 | Rabbit Anti-ELYS antibody |
| 中文名稱 | 轉(zhuǎn)錄因子ELYS蛋白抗體 |
| 別 名 | ahctf1; AT hook containing transcription factor 1; DKFZp434N093; ELYS; AT-hook-containing transcription factor 1; ELYS transcription factor like protein TMBS62; ELYS_HUMAN; Embryonic large molecule derived from yolk sac; MST108; MSTP108; Protein ELYS; Protein MEL-28; Putative AT-hook-containing transcription factor 1; TMBS62. |
| 研究領(lǐng)域 | 心血管 干細胞 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 252kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ELYS: 451-550/2266 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ELYS is a protein of the nuclear matrix that contains one AT hook DNA-binding domain. ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division. Nuclear pores span the nuclear envelope and act as gated aqueous channels to regulate the transport of macromolecules between the nucleus and cytoplasm, from individual proteins and RNAs to entire viral genomes. The A.T. hook near the C-terminus of likely indicates ELYS can function as a transcription factor which may play a specific role in hematopoietic tissues. It also may play a role in early embryo development, as ELYS is critical for the survival of inner cells of the blastocyst. Function: Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis. Subunit: Associates with the Nup107-160 subcomplex of the NPC. Subcellular Location: Cytoplasm. Nucleus matrix. Chromosome; centromere; kinetochore. Nucleus; nucleoplasm. Nucleus; nuclear pore complex. Localizes to the nuclear pore complex (NPC) throughout interphase. Localizes to the kinetochore from prophase, and this appears to require the Nup107-160 subcomplex of the NPC. Localizes to the periphery of chromatin from late anaphase. Similarity: Belongs to the ELYS family. Contains 1 A.T hook DNA-binding domain. SWISS: Q8WYP5 Gene ID: 25909 Database links: Entrez Gene: 25909 Human Omim: 610853 Human SwissProt: Q8IZA4 Human SwissProt: Q8WYP5 Human Unigene: 300887 Human |
| 產(chǎn)品圖片 |
Sample:
Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti- ELYS (bs-9880R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 252 kD
Observed band size: 252 kD
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELYS) Polyclonal Antibody, Unconjugated (bs-9880R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
