| 產(chǎn)品編號 | bs-9934R |
| 英文名稱 | Rabbit Anti-C11ORF77 antibody |
| 中文名稱 | 11號染色體開放閱讀框77抗體 |
| 別 名 | Chromosome 11 open reading frame 77; FLJ32675; HARB1_HUMAN; HARBI1; Harbinger transposase derived 1; Harbinger transposase-derived nuclease; Putative nuclease HARBI1. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 細(xì)胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human C11ORF77/HARBI1: 11-100/349 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
HARBI1 is a 349 amino acid nuclear and cytoplasmic protein belonging to the HARBI1 family. Members of the HARBI1 family of proteins are highly conserved in humans to various bony fish. Considered a transposase-derived protein, HARBI1 may possess nuclease activity and is expressed in brain, eye, nerve tissue, kidney and lung. HARBI1 utilizes divalent metal cations as cofactors, interacts with NAIF1 and promotes translocation to the nucleus. HARBI1 is encoded by a gene located on human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11. Function: Transposase-derived protein that may have nuclease activity (Potential). Does not have transposase activity. Subunit: Interacts with NAIF1. Subcellular Location: Nucleus. Cytoplasm. Interaction with NAIF1 promotes translocation to the nucleus. Tissue Specificity: Detected in brain, eye, nerve tissue, kidney and lung. Similarity: Belongs to the HARBI1 family. SWISS: Q96MB7 Gene ID: 283254 Database links: Entrez Gene: 283254 Human Entrez Gene: 241547 Mouse SwissProt: Q96MB7 Human SwissProt: Q8BR93 Mouse Unigene: 714463 Human Unigene: 130331 Mouse Unigene: 198635 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C11ORF77) Polyclonal Antibody, Unconjugated (bs-9934R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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