| 產(chǎn)品編號 | bs-9971R |
| 英文名稱 | Rabbit Anti-CREPT antibody |
| 中文名稱 | 腫瘤高表達細胞周期相關(guān)蛋白抗體 |
| 別 名 | C20orf77; Cell cycle related and expression elevated protein in tumor; Chromosome 20 open reading frame 77; CREPT; dJ1057B20.2; DKFZp434P0735; FLJ44520; Hypothetical protein LOC58490; Uncharacterized protein C20orf77; RPR1B_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 細胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Chicken) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CREPT: 221-320/326 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Interacts with phosphorylated C-terminal heptapeptide
repeat domain (CTD) of the largest RNA polymerase II subunit
POLR2A, and participates in dephosphorylation of the CTD.
Transcriptional regulator which enhances expression of CCND1.
Promotes binding of RNA polymerase II to the CCDN1 promoter and to
the termination region before the poly-A site but decreases its
binding after the poly-A site. Prevents RNA polymerase II from
reading through the 3' end termination site and may allow it to be
recruited back to the promoter through promotion of the formation
of a chromatin loop. Also enhances the transcription of a number of
other cell cycle-related genes including CDK2, CDK4, CDK6 and
cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation. Function: Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation. Subunit: Associates with the RNA polymerase II complex. Subcellular Location: Nucleus. Tissue Specificity: Preferentially expressed in a range of tumor tissues including colon, lung, liver, breast, prostate, stomach, uterine endometrium and cervical cancers with higher levels in tumors than in adjacent non-tumor tissue (at protein level). Similarity: Belongs to the UPF0400 (RTT103) family. Contains 1 CID domain. SWISS: Q9NQG5 Gene ID: 58490 Database links: Entrez Gene: 58490 Human Entrez Gene: 70470 Mouse Omim: 614694 Human SwissProt: Q9NQG5 Human SwissProt: Q9CSU0 Mouse Unigene: 278839 Human |
| 產(chǎn)品圖片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- CREPT (bs-9971R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
