| 產(chǎn)品編號(hào) | bs-11831R |
| 英文名稱 | Rabbit Anti-Chordin antibody |
| 中文名稱 | 原腸胚雙向形成相關(guān)蛋白抗體 |
| 別 名 | Chordin; Chrd; CHRD_HUMAN; MGC133038; Shortgastrulation protein; SOG. |
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Specific References (1) | bs-11831R has been referenced in 1 publications.
[IF=7.815] Zhang Y et al. Directed Differentiation of Notochord-like and Nucleus Pulposus-like Cells Using Human Pluripotent Stem Cells. Cell Rep. 2020 Feb 25;30(8):2791-2806.e5. WB ; human.
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| 研究領(lǐng)域 | 細(xì)胞生物 發(fā)育生物學(xué) 神經(jīng)生物學(xué) 干細(xì)胞 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 99kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Chordin: 412-490/951 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The patterning of the CNS relies on the interaction of multiple signaling molecules such as Sonic Hedgehog, Wnts, and BMPs and their antagonists Chordin and Noggin. At midgastrula, expression of Noggin overlaps that of Chordin. Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. The gene which encodeschordin maps to human chromosome 3q27. Noggin is a secreted protein that binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily of signaling proteins, such as BMP-2,4,7. The gene which encodes noggin maps to human chromosome 17q22. Function: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. Subunit: Interacts with TWSG1 and/or BMP4 Subcellular Location: Secreted. Tissue Specificity: Expressed at the highest level in liver. Post-translational modifications: Cleaved by tolloid proteases; cleavage participates to dorsoventral patterning during early development. Similarity: Belongs to the chordin family. Contains 4 CHRD domains. Contains 4 VWFC domains. SWISS: Q9H2X0 Gene ID: 8646 Database links: Entrez Gene: 8646 Human Entrez Gene: 12667 Mouse Omim: 603475 Human SwissProt: Q9H2X0 Human SwissProt: Q9Z0E2 Mouse Unigene: 166186 Human Unigene: 20457 Mouse Unigene: 29459 Rat |
| 產(chǎn)品圖片 |
Sample:
HepG2(Human) Cell Lysate at 30 ug
U2OS(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Chordin (bs-11831R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 96 kD
Sample:
HepG2(Human) Cell Lysate at 30 ug
U2OS(Human) Cell Lysate at 30 ug
MDA-MB-231(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Chordin (bs-11831R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 99 kD
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