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Rabbit Anti-SPOCK2  antibody (bs-11966R)  
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產(chǎn)品編號 bs-11966R
英文名稱 Rabbit Anti-SPOCK2  antibody
中文名稱 睪丸蛋白聚糖2抗體
別    名 Kazal-like domains proteoglycan 2; CWCV; FLJ97039; KIAA0275; SPARC/osteonectin; Sparc/osteonectin cwcv and kazal like domains proteoglycan (testican) 2; Sparc/osteonectin cwcv and kazal like domains proteoglycan 2; SPOCK 2; Spock2; Testican 2; Testican-2; Testican2; TICN 2; TICN2; TICN2_HUMAN.  
Specific References  (1)     |     bs-11966R has been referenced in 1 publications.
[IF=17.694] Xu An. et al. Rewired m6A epitranscriptomic networks link mutant p53 to neoplastic transformation. NAT COMMUN. 2023 Mar;14(1):1-19  IF ;  Mouse.  
研究領(lǐng)域 細(xì)胞生物  神經(jīng)生物學(xué)  細(xì)胞骨架  細(xì)胞外基質(zhì)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Mouse,Rat (predicted: Human,Chicken,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 45kDa
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SPOCK2/Testican 2: 61-160/424 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a protein which binds with glycosaminoglycans to form part of the extracellular matrix. The protein contains thyroglobulin type-1, follistatin-like, and calcium-binding domains, and has glycosaminoglycan attachment sites in the acidic C-terminal region. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Function:
May participate in diverse steps of neurogenesis. Binds calcium.

Subcellular Location:
Secreted.

Tissue Specificity:
Highly expressed in brain. Also found in lung and testis.

Post-translational modifications:
Contains chondroitin sulfate and heparan sulfate O-linked oligosaccharides.

Similarity:
Contains 1 Kazal-like domain.
Contains 1 thyroglobulin type-1 domain.

SWISS:
Q92563

Gene ID:
9806

Database links:

Entrez Gene: 9806 Human

Omim: 607988 Human

SwissProt: Q92563 Human

Unigene: 523009 Human



產(chǎn)品圖片
Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-SPOCK2 (bs-11966R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPOCK2) Polyclonal Antibody, Unconjugated (bs-11966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPOCK2) Polyclonal Antibody, Unconjugated (bs-11966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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