| 產(chǎn)品編號 | bs-7175R |
| 英文名稱 | Rabbit Anti-MAL2 antibody |
| 中文名稱 | T淋巴細(xì)胞分化蛋白MAL2抗體 |
| 別 名 | MAL 2; MAL proteolipid protein 2; Mal, T cell differentiation protein 2; mal2; MAL2_HUMAN; Protein MAL2. |
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Specific References (3) | bs-7175R has been referenced in 3 publications.
[IF=9.423] Jaekwang Jeong. et al. MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells. Cell Rep. 2021 Dec;37:110160 IF ; Human.
[IF=7.919] Le Tao. et al. FTO modifies the m6A level of MALAT and promotes bladder cancer progression. Clin Transl Med. 2021 Feb;11(2):e310 IHC ; Human.
[IF=5.875] Feiyang Deng. et al. Lipid raft-mediated and upregulated coordination pathways assist transport of glycocholic acid-modified nanoparticle in a human breast cancer cell line of SK-BR-3. Int J Pharmaceut. 2022 Apr;617:121589 IF ; Human.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號轉(zhuǎn)導(dǎo) 細(xì)胞分化 淋巴細(xì)胞 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Pig,Cow) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 19kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAL2: 21-120/176 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The MARVEL domain is a 130 amino acid motif that contains four transmembrane helices, both of which have cytoplasmic N- and C-terminal regions. MARVEL domain-containing proteins are thought to participate in tight junction regulation, the biogenesis of vesicular transport carriers and in cholesterol-rich membrane apposition events. MAL2 (mal, T-cell differentiation protein 2) is a 176 amino acid multi-pass membrane protein that is associated with lipid rafts and contains one MARVEL domain. Expressed predominately in liver, kidney and lung, MAL2 functions as a member of the polarized machinery transport system and is required for transcytosis, a transporter pathway used to deliver membrane-bound cargo from perinuclear endosomes to the apical surface in a raft-dependent manner. Differential expression of MAL2 is associated with several cancers, including renal cell carcinoma and childhood leukemia, suggesting a role for MAL2 in tumorigensis. Function: Member of the machinery of polarized transport. Required for the indirect transcytotic route at the step of the egress of the transcytosing cargo from perinuclear endosomes in order for it to travel to the apical surface via a raft-dependent pathway. Subunit: Interacts with TPD52L2. Subcellular Location: Cell membrane. Apical cell membrane. Endomembrane system. Cytoplasm > perinuclear region. Associated with lipid rafts. In polarized epithelial cells, restricted to the apical surface. In hepatocytes, as well as in polarized hepatoma HepG2 cells, found in the canalicular membrane, equivalent to the apical surface, beneath the canalicular actin cytoskeleton. In non-polarized HepG2 cells, distributed to the perinuclear region. Tissue Specificity: Predominantly expressed in kidney, lung, and liver. Also found in thyroid gland, stomach and, at lower levels in testis and small intestine. Similarity: Belongs to the MAL family. Contains 1 MARVEL domain. SWISS: Q969L2 Gene ID: 114569 Database links: Entrez Gene: 114569 Human Entrez Gene: 105853 Mouse Omim: 609684 Human SwissProt: Q969L2 Human SwissProt: Q8BI08 Mouse Unigene: 201083 Human Unigene: 410994 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAL2) Polyclonal Antibody, Unconjugated (bs-7175R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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