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Rabbit Anti-Ghrelin 28 (n-octanoyl Ser26)  antibody (bs-10413R)  
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產(chǎn)品編號 bs-10413R
英文名稱 Rabbit Anti-Ghrelin 28 (n-octanoyl Ser26)  antibody
中文名稱 辛?;X腸肽抗體
別    名 Ghrelin 28 [Ser26 (n-octanoyl)]; Appetite regulating hormone; Ghrelin 27; Ghrelin 28; Ghrelin/obestatin prepropeptide; GHRL; Growth hormone releasing peptide; Growth hormone secretagogue; M46 protein; Motilin related peptide; MTLRP; Obestatin; Obestatin preprohormone; PRO1066; UNQ524; GHRL_HUMAN.  
研究領(lǐng)域 心血管  神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)  生長因子和激素  糖尿病  新陳代謝  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 45728kDa
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised n-octanoyl-peptide derived from human Ghrelin 28 around the n-octanoyl site of Ser26): GS(n-octanoyl-S)FL 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq, Oct 2008].

Function:
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.

Subcellular Location:
Secreted.

Tissue Specificity:
Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.

Post-translational modifications:
O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
Amidation of Leu-98 is essential for obestatin activity.

Similarity:
Belongs to the motilin family.

SWISS:
Q9UBU3

Gene ID:
51738

Database links:

Entrez Gene: 51738 Human

Entrez Gene: 58991 Mouse

Entrez Gene: 59301 Rat

Entrez Gene: 574280 Rhesus monkey

Omim: 605353 Human

SwissProt: Q6BEG6 Cat

SwissProt: Q9BEF8 Dog

SwissProt: Q9UBU3 Human

SwissProt: Q9EQX0 Mouse

SwissProt: Q9GKY5 Pig

SwissProt: Q9QYH7 Rat

Unigene: 590080 Human

Unigene: 672979 Human

Unigene: 379095 Mouse

Unigene: 42103 Rat



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ghrelin 28 (n-octanoyl Ser26)) Polyclonal Antibody, Unconjugated (bs-10413R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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