| 產(chǎn)品編號(hào) | bs-14051R |
| 英文名稱 | Rabbit Anti-CREB3L1 antibody |
| 中文名稱 | 環(huán)腺苷酸應(yīng)答元件結(jié)合蛋白3樣蛋白1抗體 |
| 別 名 | BBF-2 homolog; CAMP responsive element binding protein 3 like 1; cAMP-responsive element-binding protein 3-like protein 1; CR3L1_HUMAN; Creb3l1; Cyclic AMP responsive element binding protein 3 like protein 1; OASIS; Old astrocyte specifically-induced substance; Processed cyclic AMP-responsive element-binding protein 3-like protein 1. |
| 研究領(lǐng)域 | 細(xì)胞生物 染色質(zhì)和核信號(hào) 信號(hào)轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CREB3L1: 251-350/519 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is normally found in the membrane of the endoplasmic reticulum (ER). However, upon stress to the ER, the encoded protein is cleaved and the released cytoplasmic transcription factor domain translocates to the nucleus. There it activates the transcription of target genes by binding to box-B elements. [provided by RefSeq, Jun 2013] Function: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Specifically involved in ER-stress response in astrocytes in the central nervous system (By similartity). May play a role in gliosis. In vitro, binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE. Subcellular Location: Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. Tissue Specificity: Ubiquitously expressed with high levels in pancreas and prostate. Expressed at relatively lower levels in brain. Post-translational modifications: Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is performed sequentially by site-1 and site-2 proteases (PS1 and PS2) and is triggered by translocation to the Golgi apparatus. Similarity: Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. SWISS: Q96BA8 Gene ID: 90993 Database links: Entrez Gene: 90993 Human Entrez Gene: 26427 Mouse SwissProt: Q96BA8 Human SwissProt: Q9Z125 Mouse Unigene: 405961 Human Unigene: 10353 Mouse Unigene: 24541 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CREB3L1) Polyclonal Antibody, Unconjugated (bs-14051R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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