| 產(chǎn)品編號 | bs-14053R |
| 英文名稱 | Rabbit Anti-CREB5 antibody |
| 中文名稱 | 環(huán)腺苷酸應(yīng)答元件結(jié)合蛋白5抗體 |
| 別 名 | cAMP response element binding protein 5; cAMP response element binding protein CRE Bpa; cAMP response element-binding protein CREBPa; cAMP responsive element binding protein 5; cAMP responsive element binding protein 5 isoform alpha; cAMP-responsive element-binding protein 5; CRE BPA; CRE-BPa; CREB 5; CREB-5; CREB5; CREB5_HUMAN; CREBPA; cyclic AMP responsive element binding protein 5; Cyclic AMP-responsive element-binding protein 5. |
| 研究領(lǐng)域 | 細胞生物 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 鋅指蛋白 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CREB5: 21-120/508 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The product of this gene belongs to the CRE (cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The encoded protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Function: Binds to the cAMP response element and activates transcription. Subunit: Binds DNA as a homodimer or as a heterodimer with JUN or ATF2/CREBP1. Subcellular Location: Nucleus. Similarity: Belongs to the bZIP family. Contains 1 bZIP domain. Contains 1 C2H2-type zinc finger. SWISS: Q02930 Gene ID: 9586 Database links: Entrez Gene: 9586 Human Entrez Gene: 231991 Mouse SwissProt: Q02930 Human SwissProt: Q8K1L0 Mouse Unigene: 437075 Human Unigene: 321138 Mouse Unigene: 210235 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human MCF-7 cell lysates
Primary: Anti-CREB5 (bs-14053R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa
Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CREB5 ) Polyclonal Antibody, Unconjugated (bs-14053R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CREB5) Polyclonal Antibody, Unconjugated (bs-14053R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
