| 產(chǎn)品編號 | bs-16751R |
| 英文名稱 | Rabbit Anti-KLC3 antibody |
| 中文名稱 | 驅(qū)動蛋白輕鏈3抗體 |
| 別 名 | Kinesin light chain 2; Kinesin light chain 3; KLC2; KLC2 like; KLC2-like; KLC2L; KLC3; KLC3_HUMAN; KLCt; KNS2B. |
| 研究領域 | 細胞生物 發(fā)育生物學 信號轉(zhuǎn)導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Cow,Horse,Orangutan) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLC3: 401-504/504 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the kinesin light chain gene family. Kinesins are molecular motors involved in the transport of cargo along microtubules, and are composed of two kinesin heavy chain (KHC) and two kinesin light chain (KLC) molecules. KLCs are thought to typically be involved in binding cargo and regulating kinesin activity. In the rat, a protein similar to this gene product is expressed in post-meiotic spermatids, where it associates with structural components of sperm tails and mitochondria. [provided by RefSeq, Jul 2008] Function: Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Subcellular Location: Cytoplasm > cytoskeleton. Elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria. Similarity: Belongs to the kinesin light chain family. Contains 5 TPR repeats. SWISS: Q6P597 Gene ID: 147700 Database links: Entrez Gene: 147700 Human Entrez Gene: 232943 Mouse Entrez Gene: 100173098 Orangutan Omim: 601334 Human SwissProt: Q6P597 Human SwissProt: Q91W40 Mouse SwissProt: Q5R8E2 Orangutan Unigene: 298079 Human Unigene: 213403 Mouse Unigene: 161815 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLC3) Polyclonal Antibody, Unconjugated (bs-16751R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
