| 產(chǎn)品編號 | bs-16804R |
| 英文名稱 | Rabbit Anti-KPNA4 antibody |
| 中文名稱 | KPNA4蛋白抗體 |
| 別 名 | FLJ31113; IMA4_HUMAN; Importin alpha 3; Importin alpha 4 subunit; Importin alpha Q1; Importin subunit alpha 3; Importin subunit alpha-4; IPO A3; IPOA 3; IPOA3; Karyopherin alpha 4 (importin alpha 3); Karyopherin alpha 4; Karyopherin alpha 4 importin alpha 3; Karyopherin alpha 4 subunit; Karyopherin subunit alpha 4; Karyopherin subunit alpha-4; KPNA 4; Kpna4; MGC12217; MGC26703; OTTHUMP00000213880; QIP 1; Qip1; Qip1 protein; SRP 3; SRP3. |
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Specific References (1) | bs-16804R has been referenced in 1 publications.
[IF=4.175] Junmin Li. et al. Circ_ZFR contributes to the paclitaxel resistance and progression of non-small cell lung cancer by upregulating KPNA4 through sponging miR-195-5p. Cancer Cell Int. 2021 Dec;21(1):1-15 WB ; Human.
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| 研究領(lǐng)域 | 細(xì)胞生物 染色質(zhì)和核信號 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Cow,Chicken,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KPNA4: 1-100/521 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq, Jul 2008] Function: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a nonclassical NLS. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes. Similarity: Belongs to the importin alpha family. Contains 10 ARM repeats. Contains 1 IBB domain. SWISS: O00629 Gene ID: 3840 Database links: Entrez Gene: 3840 Human Entrez Gene: 100171548 Orangutan Entrez Gene: 100349259 Rabbit Entrez Gene: 702486 Rhesus monkey Omim: 602970 Human SwissProt: O00629 Human Unigene: 288193 Human |
| 產(chǎn)品圖片 |
Sample: heart (mouse) Lysate at 40 ug
Primary: Anti-KPNA4(bs-16804R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
25 ug total protein per lane of various lysates (see on figure) probed with KPNA4 polyclonal antibody, unconjugated (bs-16804R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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