| 產(chǎn)品編號 | bs-17339R |
| 英文名稱 | Rabbit Anti-hnRNP A0 antibody |
| 中文名稱 | 異質核糖核蛋白A0抗體 |
| 別 名 | HNRNPA0; Heterogeneous nuclear ribonucleoprotein A0; hnRNA binding protein; hnRNP A0; HNRNPA0; ROA0_HUMAN. |
| 研究領域 | 細胞生物 結合蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog, Cynomolgus Monkey,Rhesus monkey,Gorilla ,Marmoset (common)) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human hnRNP A0: 1-100/305 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein components are localized to the nucleus; however, some shuttle between the nucleus and the cytoplasm. hnRNP I, also designated polypyrimidine tract-binding protein (PTB), and its homolog hnRNP L, bind to the 3' end of introns to modulate alternative splicing mechanisms of pre-mRNAs in normal cells and the translation of sev-eral viruses, including hepatitis C virus (HCV). The human hnRNP I gene maps to chromosome 19p13.3 and encodes a protein that is localized in the nucleoplasm. hnRNP L, like hnRNP I, is also localized in the nucleoplasm. Function: This protein is a component of ribonucleosomes. Subcellular Location: Nucleus. Component of ribonucleosomes. Post-translational modifications: Arg-291 is dimethylated, probably to asymmetric dimethylarginine. Similarity: Contains 2 RRM (RNA recognition motif) domains. SWISS: Q13151 Gene ID: 10949 Database links: Entrez Gene: 416302 Chicken Entrez Gene: 745876 Chimpanzee Entrez Gene: 10949 Human Entrez Gene: 77134 Mouse Omim: 609409 Human SwissProt: Q13151 Human SwissProt: Q9CX86 Mouse Unigene: 645902 Human Unigene: 96996 Human Unigene: 490274 Mouse |
| 產(chǎn)品圖片 |
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-hnRNP A0 (bs-17339R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
