| 產(chǎn)品編號 | bs-8809R |
| 英文名稱 | Rabbit Anti-HMG4/HMGB3 antibody |
| 中文名稱 | 高遷移率家族蛋白3抗體 |
| 別 名 | High mobility group (nonhistone chromosomal) protein 4; High mobility group box 3; High mobility group protein 2a; High mobility group protein 4; High mobility group protein B3; High mobility group protein HMG4; HMG 4; HMG-2a; HMG-4; HMG2A; HMGB 3; HMGB3; HMGB3_HUMAN; MGC90319; Non histone chromosomal protein; Nonhistone chromosomal protein HMG4. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 轉(zhuǎn)錄調(diào)節(jié)因子 結(jié)合蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HMG4/HMGB3: 1-100/200 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM, Mar 2008] Function: Binds preferentially single-stranded DNA and unwinds double stranded DNA. Subcellular Location: Nucleus. Chromosome. Tissue Specificity: Expressed predominantly in placenta. Similarity: Belongs to the HMGB family. Contains 2 HMG box DNA-binding domains. SWISS: O15347 Gene ID: 3149 Database links: Entrez Gene: 3149 Human Entrez Gene: 15354 Mouse Omim: 300193 Human SwissProt: O15347 Human SwissProt: O54879 Mouse Unigene: 19114 Human Unigene: 336087 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMG4) Polyclonal Antibody, Unconjugated (bs-8809R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HMG4) Polyclonal Antibody, Unconjugated (bs-8809R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
