| 產品編號 | bs-19016R |
| 英文名稱 | Rabbit Anti-NAPG antibody |
| 中文名稱 | 可溶性NSF附著蛋白γ-SNAP抗體 |
| 別 名 | Gamma SNAP; Gamma-soluble NSF attachment protein; GAMMASNAP; N ethylmaleimide sensitive factor attachment protein gamma; N-ethylmaleimide-sensitive factor attachment protein gamma; napG; SNAG_HUMAN; SNAP gamma; SNAP-gamma; Soluble NSF attachment protein; Soluble NSF-attachment protein, gamma. |
| 研究領域 | 細胞生物 神經(jīng)生物學 信號轉導 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NAPG: 121-220/312 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes soluble NSF attachment protein gamma. The soluble NSF attachment proteins (SNAPs) enable N-ethyl-maleimide-sensitive fusion protein (NSF) to bind to target membranes. NSF and SNAPs appear to be general components of the intracellular membrane fusion apparatus, and their action at specific sites of fusion must be controlled by SNAP receptors particular to the membranes being fused. The product of this gene mediates platelet exocytosis and controls the membrane fusion events of this process.[provided by RefSeq, Dec 2008] Function: Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. Subcellular Location: Membrane. Similarity: Belongs to the SNAP family. SWISS: Q99747 Gene ID: 8774 Database links: Entrez Gene: 8774 Human Entrez Gene: 108123 Mouse Omim: 603216 Human SwissProt: Q99747 Human SwissProt: Q9CWZ7 Mouse Unigene: 464622 Human Unigene: 292687 Mouse |
| 產品圖片 |
Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- NAPG (bs-19016R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 34 kD
Sample: Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- NAPG (bs-19016R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 34 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NAPG) Polyclonal Antibody, Unconjugated (bs-19016R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NAPG) Polyclonal Antibody, Unconjugated (bs-19016R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
