| 產(chǎn)品編號 | bs-21048R |
| 英文名稱 | Rabbit Anti-POU5F2 antibody |
| 中文名稱 | POU5F2蛋白抗體 |
| 別 名 | 1700013G10Rik; class 5; DKFZp686P02123; PO5F2_HUMAN; POU domain; POU domain class 5, transcription factor 2; POU5F2; Sperm 1 POU domain transcription factor; Sperm associated POU domain protein 1; SPRM 1; SPRM-1; SPRM1; transcription factor 2. |
| 研究領域 | 細胞生物 發(fā)育生物學 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human POU5F2: 211-310/328 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
POU5F2 (POU Domain Class 5, Transcription Factor 2) is a Protein Coding gene. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and sequence-specific DNA binding. An important paralog of this gene is POU5F1. Function: Transcription factor that binds preferentially to the octamer motif (5'-ATGTTAAT-3'). May exert a regulatory function in meiotic events that are required for terminal differentiation of male germ cell (By similarity). Subcellular Location: Nucleus. Tissue Specificity: Expressed in skeletal and cardiac muscles, brain, heart and lung. Little or no detectable expression found in pancreas, kidney, liver or placenta. Similarity: Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. SWISS: Q8N7G0 Gene ID: 134187 Database links: Entrez Gene: 134187 Human Entrez Gene: 75507 Mouse SwissProt: Q8N7G0 Human SwissProt: Q9DAC9 Mouse Unigene: 678995 Human Unigene: 443255 Mouse |
| 產(chǎn)品圖片 |
Sample:
A673(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Primary: Anti-POU5F2 (bs-21048R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 34 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
