| 產(chǎn)品編號 | bs-12611R |
| 英文名稱 | Rabbit Anti-PECI antibody |
| 中文名稱 | PECI蛋白抗體 |
| 別 名 | 2-trans-enoyl-CoA isomerase; ACBD2; acyl Coenzyme A binding domain containing 2; D2-enoyl-CoA isomerase; D3; D3 D2 enoyl CoA isomerase; DBI related protein 1; DBI-related protein 1; delta(2)-enoyl-CoA isomerase; Delta(3); Delta(3) delta(2) enoyl CoA isomerase; Diazepam-binding inhibitor-related protein 1; Dodecenoyl CoA isomerase; Dodecenoyl-CoA isomerase; DRS 1; DRS-1; DRS1; Eci2; ECI2_HUMAN; Enoyl-CoA delta isomerase 2; HCA88; Hepatocellular carcinoma associated antigen 88; Hepatocellular carcinoma-associated antigen 88; mitochondrial; pECI; Peroxisomal 3; Peroxisomal 3,2-trans-enoyl-CoA isomerase; Peroxisomal D3 D2 enoyl CoA isomerase; Renal carcinoma antigen NY REN 1; Renal carcinoma antigen NY-REN-1。 |
| 研究領域 | 腫瘤 細胞生物 結(jié)合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat (predicted: Human,Mouse,Pig,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PECI: 211-310/394 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2011] Function: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates. Subcellular Location: Mitochondrion and Peroxisome matrix. Tissue Specificity: Abundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells. Similarity: In the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. Contains 1 ACB (acyl-CoA-binding) domain. SWISS: O75521 Gene ID: 10455 Database links: Entrez Gene: 10455 Human Entrez Gene: 23986 Mouse Omim: 608024 Human SwissProt: O75521 Human SwissProt: Q9WUR2 Mouse Unigene: 15250 Human Unigene: 28883 Mouse Unigene: 108029 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PECI) Polyclonal Antibody, Unconjugated (bs-12611R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
