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Rabbit Anti-RBM33  antibody (bs-21295R)  
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產(chǎn)品編號(hào) bs-21295R
英文名稱 Rabbit Anti-RBM33  antibody
中文名稱 RNA結(jié)合蛋白33抗體
別    名 hypothetical protein MGC20460; proline rich 8; Proline-rich protein 8; PRR8; Rbm33; RBM33_HUMAN; RNA-binding motif protein 33; RNA-binding protein 33.  
Specific References  (1)     |     bs-21295R has been referenced in 1 publications.
[IF=10.75] Zhong Chuanfan. et al. M6A-modified circRBM33 promotes prostate cancer progression via PDHA1-mediated mitochondrial respiration regulation and presents a potential target for ARSI therapy. INT J BIOL SCI. 2023 Mar;19(5):1543-1563  WB ;  Human.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  結(jié)合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 130kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human RBM33: 51-150/1170 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹
產(chǎn)品圖片
Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- RBM33 (bs-21295R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBM33) Polyclonal Antibody, Unconjugated (bs-21295R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBM33) Polyclonal Antibody, Unconjugated (bs-21295R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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