| 產(chǎn)品編號 | bs-23862R |
| 英文名稱 | Rabbit Anti-RGC32 antibody |
| 中文名稱 | 補(bǔ)體應(yīng)答基因32抗體 |
| 別 名 | RGC32; Chromosome 13 open reading frame 15; KIAA0564; MGC87338; response gene to complement 32; Response gene to complement 32 protein; RGC 32; RGCC_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 細(xì)胞周期蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/1ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RGC32: 31-100/137 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
RGC32, also known as C13orf15, is a 137 amino acid protein that localizes to the cytoplasm, as well as to the nucleus and the centrosome. Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart and placenta, RGC32 functions to modulate the activity of cell cycle-specific kinases, thereby regulating cell cycle progression. Additionally, RGC32 may promote cell cycle arrest at the G2/M phase transition and is thought to inhibit the growth of glioma cells, possibly functioning as a tumor suppressor. Conversely, overexpression of RGC32 may promote cell replication and assist in the pathogenesis of malignancies, suggesting that RGC32 also participates in tumor transformation and progression. RGC32 activity is induced by complement activation and by p53 in response to DNA damage. Multiple isoforms of RGC32 exist as a result of alternative splicing events. Function: Modulates the activity of cell cycle-specific kinases. Enhances CDK1 activity. May contribute to the regulation of the cell cycle. May inhibit growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. Fibrogenic factor contributing to the pathogenesis of renal fibrosis through fibroblast activation. Subunit: Interacts with SMAD3. Interacts with CDK1 and PLK1. Subcellular Location: Cytoplasm. Nucleus. Cytoplasm ?cytoskeleton ?centrosome. Note: Cytoplasmic in unstimulated cells. Nuclear after activation by complement. Associated with the centrosome during prometaphase and metaphase. Tissue Specificity: Detected in brain, heart and liver (at protein level). Highly expressed in liver, skeletal muscle, kidney and pancreas. Detected at lower levels in heart, brain and placenta. Detected in aorta endothelial cells. Overexpressed in colon, breast, prostate, bladder, lung, and ovarian cancer tissues. SWISS: Q9H4X1 Gene ID: 28984 Database links: Entrez Gene: 28984 Human Omim: 610077 Human SwissProt: Q9H4X1 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RGC32) Polyclonal Antibody, Unconjugated (bs-23862R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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