| 產(chǎn)品編號 | bs-21397R |
| 英文名稱 | Rabbit Anti-KIF3A antibody |
| 中文名稱 | 驅(qū)動蛋白家族蛋白3抗體 |
| 別 名 | KIF 3; KIF-3; KIF 3A; KIF-3A; KIF3; Kif3a; KIF3A_HUMAN; Kifl; kinesin family member 3A; Kinesin family protein 3; Kinesin family protein 3A; Kinesin like protein KIF 3A; Kinesin like protein KIF3A; Kinesin-like protein KIF3A; Kns3; Microtubule plus end directed kinesin motor 3A; Microtubule plus end-directed kinesin motor 3A. |
| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 干細胞 細胞外基質(zhì) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 80kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KIF3A |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The kinesins constitute a large family of microtubule-dependent motor proteins which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell. Individual kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events including endocytosis and transcytosis. Members of the heterotrimeric kinesin II family of microtubule associated motors generally contain two different motor subunits from the KIF3 family, which includes KIF3A, B and C. KIF3 isoforms mediate anterograde transport of membrane bound organelles in neurons and melanosomes, transport between the endoplasmic reticulum and the Golgi, and transport of protein complexes within cilia and flagella required for their morphogenesis. KIF3A may influence neurogenesis at the level of embryonic cellular events, where the asymmetry of the genetic control circuit controlling left-right (L-R) axis determination is defined. Loss of KIF3A function in mice photoreceptors causes apoptotic cell death, suggesting that kinesin II mediated transport is required for proper cell fate. Function: Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Subunit: Belongs to the kinesin-like protein family. Kinesin II subfamily. Contains 1 kinesin-motor domain. Subcellular Location: Cytoplasm, cytoskeleton (Probable). Cell projection, cilium (By similarity). Similarity: Belongs to the kinesin-like protein family. Kinesin II subfamily. Contains 1 kinesin-motor domain. SWISS: Q9Y496 Gene ID: 11127 Database links: Entrez Gene: 11127 Human Entrez Gene: 16568 Mouse Omim: 604683 Human SwissProt: Q9Y496 Human SwissProt: P28741 Mouse Unigene: 43670 Human Unigene: 88694 Mouse Unigene: 47400 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIF3A) Polyclonal Antibody, Unconjugated (bs-21397R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
