| 產品編號 | bs-22205R |
| 英文名稱 | Rabbit Anti-n-Myc antibody |
| 中文名稱 | 致癌基因n-Myc抗體 |
| 別 名 | bHLHe37; Class E basic helix-loop-helix protein 37; MODED; MYCN; MYCN_HUMAN; N myc; N myc proto oncogene protein; N-myc proto-oncogene protein; Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene; Neuroblastoma MYC oncogene; NMYC; NMYC proto oncogene protein; ODED; Oncogene NMYC; pp65/67; v myc avian myelocytomatosis viral related oncogene neuroblastoma derived; v myc myelocytomatosis viral related oncogene neuroblastoma derived. |
| 研究領域 | 腫瘤 細胞生物 免疫學 染色質和核信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human n-Myc : 1-100/464 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. [provided by RefSeq, Jul 2008]. Function: May function as a transcription factor. Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an heterodimer with MAX. Interacts with KDM5A, KDM5B and HUWE1. Subcellular Location: Nucleus. DISEASE: Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. SWISS: P04198 Gene ID: 4613 Database links: Entrez Gene: 4613 Human Entrez Gene: 18109 Mouse Omim: 164840 Human SwissProt: P04198 Human SwissProt: P03966 Mouse Unigene: 25960 Human Unigene: 16469 Mouse Unigene: 81116 Rat |
| 產品圖片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-n-Myc (bs-22205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample:
Lane 1: Human SH-SY5Y cell Lysates
Lane 2: Human Hela cell Lysates
Primary: Anti-n-Myc (bs-22205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49kDa
Observed band size: 60kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
