| 產(chǎn)品編號 | D-9112 |
| 英文名稱 | Dihydrorhodamine 123 |
| 中文名稱 | 熒光素二氫羅丹明123 |
| 別 名 | MFCD00285712; Benzoic acid, 2-(3,6-diamino-9H-xanthen-9-yl)-, methyl ester; Methyl 2-(3,6-diamino-9H-xanthen-9-yl)benzoate; DHR 123; 二氫若丹明123 |
| CAS | 109244-58-8 |
| 保存條件 | Store at -20℃, protect from light. |
| 產(chǎn)品介紹 |
Dihydrorhodamine 123 is a fluorescent probe (λex=488 nm, λem=525 nm). Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes (1). Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) (2,3) and peroxynitrite (4,5). Also see dihydrorhodamine 123 dihydrochloride, a more stable and water soluble form of dihydrorhodamine 123. Dihydrorhodamine 123 是一種熒光探針 (λex=488 nm，λem=525 nm)。 CAS：109244-58-8 分子式：C21H18N2O3 分子量：346.38 MDL：MFCD00285712 簡介：二氫羅丹明123 (Dihydrorhodamine 123)是一種不帶電荷且無熒光的活性氧(ROS)指示劑,能夠主動擴(kuò)散穿透細(xì)胞膜，進(jìn)入細(xì)胞后被氧化為陽離子的羅丹明123，定位在線粒體上，發(fā)射亮綠色熒光（Ex/Em=500/536 nm）。淡一點一氧化氮 (NO) ，超氧化物，或過氧化氫 (H2O2) 不能氧化DHR123，但是，當(dāng)這些活性氧物質(zhì) (ROS) 與其他細(xì)胞組分比如細(xì)胞色素C氧化酶或Fe2+聯(lián)合起來能夠氧化DHR123生成應(yīng)該衍生物羅丹明123.DHR 123能用了檢測內(nèi)皮細(xì)胞、嗜酸性細(xì)胞和反應(yīng)性小膠質(zhì)細(xì)胞產(chǎn)生的活性氧中間產(chǎn)物。 |
