| 產(chǎn)品編號 | bsm-51570M |
| 英文名稱 | Mouse Anti-Prohibitin antibody |
| 中文名稱 | 阻抑素/腫瘤抑制因子單克隆抗體 |
| 別 名 | Mitochondrial Marker; PHB; PHB1; Prohibitin - Mitochondrial Marker; PHB_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 染色質(zhì)和核信號 細胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 細胞類型標志物 鋅指蛋白 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | E4F12 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1000,IHC-P=1:25-50,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Prohibitin between 1-205 amino acids. |
| 亞 型 | IgG2b,k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Function: Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. Subunit: Interacts with PHB2. Interacts with STOML2. Subcellular Location: Mitochondrion inner membrane. Tissue Specificity: Widely expressed in different tissues. Similarity: Belongs to the prohibitin family. SWISS: P35232 Gene ID: 5245 Database links: Entrez Gene: 5245 Human SwissProt: P35232 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: 293 cell lysates
Lane 2: MCF-7 cell lysates
Lane 3: HepG2 cell lysates
Lane 4: NIH/3T3 cell lysates
Lane 5: Rat Liver tissue lysates
Primary: Anti-Prohibitin (bsm-51570M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Paraformaldehyde-fixed, paraffin embedded (Human stomach section); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Prohibitin) Monoclonal Antibody, Unconjugated (bsm-51570M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
