| 產(chǎn)品編號 | bs-42024R |
| 英文名稱 | Rabbit Anti-RSV N antibody |
| 中文名稱 | 呼吸道合胞病毒核蛋白抗體 |
| 別 名 | Respiratory syncytial virus nucleoprotein; N; NCAP_HRSVA; Nucleoprotein; Protein N; Nucleocapsid protein. |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | HRSVA |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43kDa |
| 細胞定位 | 細胞膜 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant HRSVA Nucleoprotein protein: 1-391/391 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Respiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. RSV is a negative sense, enveloped RNA virus. The virion is variable in shape and size with average diameter of between 120 and 300 nm. The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, results in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells. Function: Attaches the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. Interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, lacks both neuraminidase and hemagglutinating activities. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors. Subunit: Homooligomer. Interacts (via N-terminus) with protein M. Interacts with protein F; this interaction occurs on the surface of infected cells. Interacts with protein SH. Interacts with host CX3CR1; this interaction modulates host immune response. Subcellular Location: Virion membrane. Host cell surface. Isoform Secreted glycoprotein G: Secreted. Post-translational modifications: May carry 40-80 separate O-linked carbohydrate chains distributed among the 91 serine and threonine residues. Palmitoylated. Similarity: Belongs to the pneumoviruses glycoprotein G family. SWISS: P03423 Gene ID: N/A 呼吸道合胞體病毒(RSV)在全球?qū)儆谝潞粑栏腥镜某Ｒ娖鹨颍质菋胗變汉粑栏腥咀畛Ｒ姷牟≡?也是引致1歲以下嬰兒肺部受感染的最普遍原因。 |
| 產(chǎn)品圖片 |
20 ng rHRSVA Nucleoprotein protein (bs-42024P) per lane probed with RSV N polyclonal antibody respectively, unconjugated (bs-42024R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
