| 產(chǎn)品編號 | bs-33272R |
| 英文名稱 | Rabbit Anti-Alpha-actinin-1 antibody |
| 中文名稱 | α-輔肌動蛋白1抗體 |
| 別 名 | ACTN 1; ACTN1_HUMAN; Alpha Actinin 1; Alpha actinin cytoskeletal isoform; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; FLJ40884; FLJ54432; Actinin Alpha 1; F-Actin Cross-Linking Protein; Non-Muscle Alpha-Actinin-1; Actinin, Alpha 1; Actinin 1 Smooth Muscle; BDPLT15; |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 103kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Alpha-actinin-1 protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Subunit: Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP. Interacts with PSD. Interacts with MICALL2 (By similarity). Interacts with DNM2 and CTTN (By similarity). Subcellular Location: Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Cell membrane. Cell junction. Cell projection ? ruffle. Similarity: Belongs to the alpha-actinin family. Contains 1 actin-binding domain. Contains 2 CH (calponin-homology) domains. Contains 2 EF-hand domains. Contains 4 spectrin repeats. SWISS: P12814 Gene ID: 87 Database links: |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Alpha-actinin-1 polyclonal antibody, unconjugated (bs-33272R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
