| 產(chǎn)品編號(hào) | bs-1148R-Gold |
| 英文名稱1 | Rabbit Anti-Cyclin D2/Gold Conjugated antibody |
| 中文名稱 | 膠體金標(biāo)記的周期素D2抗體 |
| 別 名 | CCND 2; CCND2; CyclinD2; G1/S specific cyclin D2; KIAK0002; MGC102758; CCND2_HUMAN; G1/S-specific cyclin-D2. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買 大包裝/詢價(jià) |
| 說(shuō) 明 書 | 100ul（10nm 15nm 35nm） |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 細(xì)胞周期蛋白 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Rat, (predicted: Mouse, ) |
| 產(chǎn)品應(yīng)用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 35kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from the middle of human Cyclin D2 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產(chǎn)品介紹 |
background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Function: Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Subunit: Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Component of the ternary complex cyclin D/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity. Subcellular Location: Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. Similarity: Belongs to the cyclin family. Cyclin D subfamily. Contains 1 cyclin N-terminal domain. Database links: Entrez Gene: 894 Human Entrez Gene: 12444 Mouse Omim: 123833 Human SwissProt: P30279 Human SwissProt: P30280 Mouse Unigene: 376071 Human Unigene: 333406 Mouse Unigene: 96083 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Cyclin D2與周期素D1一樣，為細(xì)胞周期中G1期進(jìn)入S期的一個(gè)重要調(diào)控因子。細(xì)胞周期素是周期素依賴激酶的調(diào)節(jié)因子，不同的細(xì)胞周期素控制著細(xì)胞周期中不同的特定階段。Cyclin D2通過(guò)激活CdK4或CdK6等，促進(jìn)DNA合成，加速細(xì)胞增殖。主要用于B細(xì)胞淋巴瘤、乳腺癌、頭頸部鱗癌、食道癌、肝癌和肺癌等惡性腫瘤的研究。 |
