| 產品編號 | bs-0820R-AP |
| 英文名稱1 | Rabbit Anti-EBNA 3A/AP Conjugated antibody |
| 中文名稱 | 堿性磷酸酶（AP）標記的EB病毒核抗原-3A抗體 |
| 別 名 | nuclear antigen EBNA-3; Epstein-Barr nuclear antigen 3; EBV nuclear antigen 3; EBNA-3; Epstein-Barr nuclear antigen 3A; EBV nuclear antigen 3A; EBNA-3A; Epstein Barr Virus; EBV-NA3; EBNA3_EBVG. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | |
| 產品應用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 103kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human herpesvirus 4 EBNA 3A (321-345aa) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: EBNA3A is a latent viral nuclear protein expressed in Epstein Barr Virus (EBV) transformed lymphoblastic cell lines. It is also found in some immunoblastic lymphomas in vivo. This viral nuclear protein is essential for EBV mediated transformation of B lymphocytes. The EBNA3A functions as a transcriptional regulator though the target genes are currently unknown. Function: Plays an essential role for activation and immortalization of human B-cells. Represses transcription of viral promoters TP1 and Cp through interaction with host RBPJ, and inhibits EBNA2-mediated activation of these promoters. Since Cp is the promoter for all EBNA mRNAs, EBNA3A probably contributes to a negative autoregulatory control loop. Subunit: Interacts with human UCKL1. Interacts with host CTPB1; this interaction seems important for EBNA3-mediated transcriptional repression. Interacts with host RBPJ. Subcellular Location: Host nucleus matrix. Note=Associated with the nuclear matrix. Similarity: Belongs to the herpesviridae EBNA-3 family. Database links: Entrez Gene: 3783762 EBVG SwissProt: Q3KST2 EBVG Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. EB病毒核抗原-3（nuclear antigen EBNA-3）是EB病毒編碼核蛋白，位于細胞核的新的酶聚合體，主要作為轉錄調節(jié)因子，指導蛋白與蛋白之間相互作用，為核糖核苷酸補救途徑的一部分. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
