| 產(chǎn)品編號 | bs-3778R-BF488 |
| 英文名稱1 | Rabbit Anti-CTCF/BF488 Conjugated antibody |
| 中文名稱 | BF488標(biāo)記的轉(zhuǎn)錄阻抑蛋白CTCF抗體 |
| 別 名 | 11 zinc finger protein; 11 zinc finger transcriptional repressor; CCCTC binding factor (zinc finger protein); CCCTC binding factor; CTCFL paralog; Transcriptional repressor CTCF; CTCF_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 發(fā)育生物學(xué) 染色質(zhì)和核信號 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 83kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CTCF (465-510aa) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: CTCF (CCCTC-binding factor) is a highly conserved zinc finger protein that has been implicated in playing a role in a wide range of regulatory functions, including transcriptional activation/repression, insulation, imprinting, and X chromosome inactivation. A general role for CTCF in the global organisation of chromatin architecture has been proposed. It has been suggested that CTCF is involved, in a heritable manner, in the interplay between DNA methylation, higher-order chromatin structure, and lineage-specific gene expression. Function: Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. Subunit: Interacts with CHD8. Subcellular Location: Nucleus, nucleoplasm. Chromosome. Chromosome, centromere. Note=May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase. Tissue Specificity: Ubiquitous. Absent in primary spermatocytes. Post-translational modifications: Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter (By similarity). Similarity: Belongs to the CTCF zinc-finger protein family. Contains 11 C2H2-type zinc fingers. Database links: Entrez Gene: 10664 Human Entrez Gene: 13018 Mouse Omim: 604167 Human SwissProt: P49711 Human SwissProt: Q61164 Mouse Unigene: 368367 Human Unigene: 269474 Mouse Unigene: 18612 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CTCF是一種結(jié)構(gòu)保守、功能多樣的鋅指蛋白。通過不同鋅指的組合，識別不同的DNA序列，并且通過自身形成多聚體或與其它蛋白相互作用、與亞細(xì)胞核結(jié)構(gòu)共定位等多種方式，在基因活化與沉默、基因印記、X-染色體失活以及基因組核內(nèi)空間組織等不同生理過程中發(fā)揮關(guān)鍵作用。CTCF在細(xì)胞生長與增殖、系統(tǒng)發(fā)生以及腫瘤發(fā)生等方面都有著重要的作用。 |
