| 產(chǎn)品編號 | bs-9313R-Bio |
| 英文名稱1 | Rabbit Anti-MAP-9/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的微管相關蛋白9抗體 |
| 別 名 | ASAP; Aster associated protein; MAP9; MAP 9; microtubule associated protein 9; MAP9_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 信號轉(zhuǎn)導 細胞周期蛋白 細胞分化 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 產(chǎn)品應用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 74kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAP-9 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Microtubules, the primary component of the cytoskeletal network, interact with proteins called microtubule-associated proteins (MAPs).MAP9 is a microtubule-associated protein required for spindle function, mitotic progression, and cytokinesis.The microtubule-associated proteins can be divided into two groups, structural and dynamic. The MAP proteins function to stimulate tubulin assembly, enhance microtubule stability, influence the spatial distribution of microtubules within cells and utilize microtubule polarity to translocate cellular components. MAP-9 (microtubule-associated protein 9), also known as ASAP, is a 647 amino acid cytoplasmic protein that is constitutively expressed during the cell cycle. MAP-9 localizes to microtubules in interphase, associates with the mitotic spindle during mitosis and localizes to the central body during cytokinesis. Involved in organization of the bipolar mitotic spindle, MAP-9 is required for bipolar spindle assembly, mitosis progression and cytokinesis. MAP-9 may be involved in stabilizing interphase microtubules. Two isoforms of MAP-9 are produced due to alternative splicing events. Function: Involved in organization of the bipolar mitotic spindle. Required for bipolar spindle assembly, mitosis progression and cytokinesis. May act by stabilizing interphase microtubules. Subunit: Binds to purified microtubules via its C-terminus. Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Note=Localizes to microtubules in interphase, associates with the mitotic spindle during mitosis, localizes to the central body during cytokinesis. Database links: Entrez Gene: 79884 Human Omim: 610070 Human SwissProt: Q49MG5 Human Unigene: 61271 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
